Effect of melatonin on torsion and reperfusion induced pathogenesis of rat uterus

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dc.authorid0000-0001-5653-0025
dc.authorid0000-0003-1365-1729
dc.authorscopusid16242768200
dc.authorscopusid6602416499
dc.authorscopusid57194180999
dc.authorscopusid6507858649
dc.authorscopusid7004903750
dc.authorscopusid9038072400
dc.authorscopusid56412874000
dc.authorwosidAKPOLAT, Nusret/ABF-6986-2020
dc.authorwosidRisvanli, Ali/W-5667-2018
dc.contributor.authorSaat, Nevzat
dc.contributor.authorRışvanlı, Ali
dc.contributor.authorDoğan, Halef
dc.contributor.authorÖnalan, E.
dc.contributor.authorAkpolat, N.
dc.contributor.authorŞeker, İbrahim
dc.contributor.authorŞahna, E.
dc.date.accessioned2022-05-11T14:42:14Z
dc.date.available2022-05-11T14:42:14Z
dc.date.issued2019
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü, Doğum ve Jinekoloji Ana Bilim Dalı
dc.description.abstractWe investigated the use of melatonin to improve fertility and reduce uterine damage caused by torsion of the uterus in pregnant rats. We used 35 pregnant rats at gestational age 18 days. The animals were randomized into five groups. Group 1 was anesthetized only. Group 2 was subjected to experimental uterine torsion of 360 degrees and the torsion was corrected after 6 h. Group 3 was subjected to uterine torsion of 360 degrees, the torsion was corrected after 6 h and melatonin was administered at the time of correction. Group 4 rats were subjected to 360o uterine torsion and melatonin was administered 6 h later at the time of correction. Group 5 was administered melatonin followed by uterine torsion of 360 degrees followed by correction of torsion 6 h later. Samples were obtained from the uterine horns on the day 1 postpartum. We used Bax, Bcl-2 and caspase 3 staining to measure apoptosis in the uterine tissues. The mRNA levels of Rho-associated, coiled-coil containing protein kinases 1 (ROCK1), homeobox D10 (Hox4 HoxD10), TLR4, NF kappa B1, caveolin 1 (Cav1) heat shock protein 90 alpha (cytosolic), class B member 1 (Hsp90ab1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were determined using quantitative real-time polymerase chain reaction analysis (qRT-PCR). Bax, Bcl-2 and caspase 3 were detected using immunohistochemistry. No difference was observed among groups with respect to abortion, neonatal mortality or congenital abnormalities. Compared to the control group, the mRNA levels of Rock1, Hox4, TLR4, NF kappa B1, Cav1 and Hsp90 genes were decreased significantly in the study groups; the decrease was greater in groups 3 and 4, which were treated with melatonin. The greatest amount of Bax staining was found in group 1 and the least amount of Bcl-2 staining was found in groups 4 and 5; the greatest amount of caspase 3 staining was found in group 2. Our findings indicate that melatonin reduced uterine torsion related tissue damage and that its application during torsion was more effective than application following removal of torsion.
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK - 115O381]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115O381]
dc.description.sponsorshipOur study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK - 115O381); TUBITAK [115O381].
dc.identifier.doi10.1080/10520295.2019.1605456
dc.identifier.endpage539
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.issue7en_US
dc.identifier.pmid31070494
dc.identifier.scopus2-s2.0-85065662438
dc.identifier.scopusqualityQ2
dc.identifier.startpage533
dc.identifier.urihttps://doi.org/10.1080/10520295.2019.1605456
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9296
dc.identifier.volume94
dc.identifier.wosWOS:000469590100001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDoğan, Halef
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofBiotechnic & Histochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIschemia
dc.subjectmelatonin
dc.subjectrat
dc.subjectreperfusion
dc.subjecttorsion
dc.subjectuterus
dc.subjectMyocardial Ischemia-Reperfusion
dc.subjectPharmacological Concentrations
dc.subjectInfarct Size
dc.subjectInjury
dc.subjectIschemia/Reperfusion
dc.subjectArrhythmias
dc.subjectDeficiency
dc.subjectReceptors
dc.subjectProteins
dc.subjectDamage
dc.titleEffect of melatonin on torsion and reperfusion induced pathogenesis of rat uterus
dc.typeArticle

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