Construction of succinimide group substituted polythiophene polymer functionalized sensing platform for ultrasensitive detection of KLK 4 cancer biomarker

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dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:24Z
dc.date.available2022-05-11T14:07:24Z
dc.date.issued2020
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA novel impedimetric immunosensor based on conjugated succinimide group substituted polythiophene polymer (SucS-PThi) modified indium tin oxide (ITO) electrode was fabricated for ultra-sensitive and ultra-selective determination of kallikrein 4 (KLK 4) antigen in diluted human serum. The basic reason for the usage of SucS-PThi polymer was that its rich effective functional groups and biocompatibility. KLK 4, an important prostate biomarker was the target analyte and anti-KLK 4 antibodies immobilized electrode was the diagnostic tool for KLK 4 antigen detection. The fabricated biosensor was characterized by employing electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX), fourier transform-infrared (FTIR) and Raman spectroscopy. Under optimized conditions, a linear concentration range from 0.04 pg/mL to 30 pg/mL and a low detection limit (LOD) of 12.2 fg/mL were obtained and the LOD was lower than of most of the existing techniques, illustrating its feasibility for practical application. Moreover, the developed sensing system combined the outstanding properties in terms of selectivity, sensitivity, repeatability, reproducibility and reusability, without requiring for labor ? intensive labeling stages. In addition, it was successfully utilized for accurate quantification of KLK 4 antigen in human serum samples. © 2020 Elsevier B.V.
dc.description.sponsorshipNKUBAP.00; Consejo Nacional para Investigaciones Científicas y Tecnológicas, CONICIT
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council of by Tekirdağ Namık Kemal University, NKU-BAP (Grant number: NKUBAP.00.ÖNAP.19.209 ).
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council of by Tekirda? Nam?k Kemal University, NKU-BAP (Grant number: NKUBAP.00.?NAP.19.209).
dc.identifier.doi10.1016/j.snb.2020.128788
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85090007896
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.snb.2020.128788
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5094
dc.identifier.volume325
dc.identifier.wosWOS:000581627000027
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAydın, Muhammet
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofSensors and Actuators, B: Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectdisposable biosensor
dc.subjectkallikrein 4
dc.subjectsuccinimide groups substituted polythiophene polymer
dc.subjectAntigens
dc.subjectBiocompatibility
dc.subjectBiomarkers
dc.subjectChemical detection
dc.subjectConjugated polymers
dc.subjectCyclic voltammetry
dc.subjectElectrochemical impedance spectroscopy
dc.subjectElectrodes
dc.subjectReusability
dc.subjectScanning electron microscopy
dc.subjectTin oxides
dc.subjectAccurate quantifications
dc.subjectEnergy dispersive x-ray
dc.subjectFourier transform infrared
dc.subjectImpedimetric immunosensors
dc.subjectIndium tin oxide electrodes
dc.subjectOptimized conditions
dc.subjectSelective determination
dc.subjectUltrasensitive detection
dc.subjectFourier transform infrared spectroscopy
dc.titleConstruction of succinimide group substituted polythiophene polymer functionalized sensing platform for ultrasensitive detection of KLK 4 cancer biomarker
dc.typeArticle

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