Construction of succinimide group substituted polythiophene polymer functionalized sensing platform for ultrasensitive detection of KLK 4 cancer biomarker
Yükleniyor...
Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel impedimetric immunosensor based on conjugated succinimide group substituted polythiophene polymer (SucS-PThi) modified indium tin oxide (ITO) electrode was fabricated for ultra-sensitive and ultra-selective determination of kallikrein 4 (KLK 4) antigen in diluted human serum. The basic reason for the usage of SucS-PThi polymer was that its rich effective functional groups and biocompatibility. KLK 4, an important prostate biomarker was the target analyte and anti-KLK 4 antibodies immobilized electrode was the diagnostic tool for KLK 4 antigen detection. The fabricated biosensor was characterized by employing electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX), fourier transform-infrared (FTIR) and Raman spectroscopy. Under optimized conditions, a linear concentration range from 0.04 pg/mL to 30 pg/mL and a low detection limit (LOD) of 12.2 fg/mL were obtained and the LOD was lower than of most of the existing techniques, illustrating its feasibility for practical application. Moreover, the developed sensing system combined the outstanding properties in terms of selectivity, sensitivity, repeatability, reproducibility and reusability, without requiring for labor ? intensive labeling stages. In addition, it was successfully utilized for accurate quantification of KLK 4 antigen in human serum samples. © 2020 Elsevier B.V.
Açıklama
Anahtar Kelimeler
disposable biosensor, kallikrein 4, succinimide groups substituted polythiophene polymer, Antigens, Biocompatibility, Biomarkers, Chemical detection, Conjugated polymers, Cyclic voltammetry, Electrochemical impedance spectroscopy, Electrodes, Reusability, Scanning electron microscopy, Tin oxides, Accurate quantifications, Energy dispersive x-ray, Fourier transform infrared, Impedimetric immunosensors, Indium tin oxide electrodes, Optimized conditions, Selective determination, Ultrasensitive detection, Fourier transform infrared spectroscopy
Kaynak
Sensors and Actuators, B: Chemical
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
325
