A novel electrochemical immunosensor based on acetylene black/epoxy-substituted-polypyrrole polymer composite for the highly sensitive and selective detection of interleukin 6

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dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:24Z
dc.date.available2022-05-11T14:07:24Z
dc.date.issued2021
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractAn ultrasensitive immunosensor based on acetylene black (AB)/epoxy-substituted-poly(pyrrole) polymer (EpxS-PPyr) composite coated disposable indium tin oxide (ITO) electrode was fabricated for interleukin 6 (IL 6) detection. The EpxS-PPyr polymer was a promising matrix material to increase the loading capacity of immunosensor owing to its large surface area and abundance of epoxy groups. EpxS-PPyr polymer was synthesized by an esterification reaction and used to attach IL 6 receptor owing to its excellent biocompatibility and good conductivity. The electrochemical signals of the electrodes during the immunosensor fabrication were performed with electrochemical impedance spectroscopy and cyclic voltammetry techniques. Additionally, the changes formed on ITO electrode surfaces were followed by scanning electron microscopy and atomic force microscopy analyses. Under optimized conditions, the designed biosensor illustrated an ultra-sensitive signal towards IL 6 antigen at a broad concentration range from 0.01 pg/mL to 50 pg/mL. The detection limit and sensitivity were found as 3.2 fg/mL and 0.29 pg?1mLk? cm?2, respectively. Acceptable reproducibility, good storage-stability and excellent selectivity were found for IL 6 determination. Moreover, the proposed biosensor was applied to human serums and the recovery rates were ranged from 99.5 to 100.5 indicating acceptable accuracy. The results illustrated that this immunosensor were suitable for detection of IL 6 in clinical samples. © 2020 Elsevier B.V.
dc.description.sponsorshipNKUBAP.00; Consejo Nacional para Investigaciones Científicas y Tecnológicas, CONICIT
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council of Tekirda? Nam?k Kemal University, TURKEY, TNKU-BAP (Grant number: NKUBAP.00.?NAP.19.209).
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council of Tekirdağ Namık Kemal University , TURKEY, TNKU-BAP (Grant number: NKUBAP.00.ÖNAP.19.209 ).
dc.identifier.doi10.1016/j.talanta.2020.121596
dc.identifier.issn0039-9140
dc.identifier.pmid33167270
dc.identifier.scopus2-s2.0-85090425856
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2020.121596
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5097
dc.identifier.volume222
dc.identifier.wosWOS:000589941600006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcetylene black
dc.subjectElectrochemical impedance spectroscopy
dc.subjectEpoxy-substituted-polypyrrole polymer
dc.subjectAcetylene
dc.subjectAtomic force microscopy
dc.subjectBiocompatibility
dc.subjectCarbon black
dc.subjectCyclic voltammetry
dc.subjectElectrochemical impedance spectroscopy
dc.subjectEsterification
dc.subjectImmunosensors
dc.subjectIndium compounds
dc.subjectLighting
dc.subjectPolymers
dc.subjectScanning electron microscopy
dc.subjectTin oxides
dc.subjectConcentration ranges
dc.subjectElectrochemical immunosensors
dc.subjectElectrochemical signals
dc.subjectEsterification reactions
dc.subjectIndium tin oxide electrodes
dc.subjectLoading capacities
dc.subjectOptimized conditions
dc.subjectSelective detection
dc.subjectElectrochemical electrodes
dc.subjectacetylene
dc.subjectimmobilized antibody
dc.subjectinterleukin 6
dc.subjectpolymer
dc.subjectpolypyrrole
dc.subjectpyrrole derivative
dc.subjectAfrican American
dc.subjectelectrochemical analysis
dc.subjectelectrode
dc.subjectgenetic procedures
dc.subjecthuman
dc.subjectimmunoassay
dc.subjectreproducibility
dc.subjectAcetylene
dc.subjectAfrican Americans
dc.subjectAntibodies, Immobilized
dc.subjectBiosensing Techniques
dc.subjectElectrochemical Techniques
dc.subjectElectrodes
dc.subjectHumans
dc.subjectImmunoassay
dc.subjectInterleukin-6
dc.subjectPolymers
dc.subjectPyrroles
dc.subjectReproducibility of Results
dc.titleA novel electrochemical immunosensor based on acetylene black/epoxy-substituted-polypyrrole polymer composite for the highly sensitive and selective detection of interleukin 6
dc.typeArticle

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