A novel electrochemical immunosensor based on acetylene black/epoxy-substituted-polypyrrole polymer composite for the highly sensitive and selective detection of interleukin 6
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An ultrasensitive immunosensor based on acetylene black (AB)/epoxy-substituted-poly(pyrrole) polymer (EpxS-PPyr) composite coated disposable indium tin oxide (ITO) electrode was fabricated for interleukin 6 (IL 6) detection. The EpxS-PPyr polymer was a promising matrix material to increase the loading capacity of immunosensor owing to its large surface area and abundance of epoxy groups. EpxS-PPyr polymer was synthesized by an esterification reaction and used to attach IL 6 receptor owing to its excellent biocompatibility and good conductivity. The electrochemical signals of the electrodes during the immunosensor fabrication were performed with electrochemical impedance spectroscopy and cyclic voltammetry techniques. Additionally, the changes formed on ITO electrode surfaces were followed by scanning electron microscopy and atomic force microscopy analyses. Under optimized conditions, the designed biosensor illustrated an ultra-sensitive signal towards IL 6 antigen at a broad concentration range from 0.01 pg/mL to 50 pg/mL. The detection limit and sensitivity were found as 3.2 fg/mL and 0.29 pg?1mLk? cm?2, respectively. Acceptable reproducibility, good storage-stability and excellent selectivity were found for IL 6 determination. Moreover, the proposed biosensor was applied to human serums and the recovery rates were ranged from 99.5 to 100.5 indicating acceptable accuracy. The results illustrated that this immunosensor were suitable for detection of IL 6 in clinical samples. © 2020 Elsevier B.V.
Açıklama
Anahtar Kelimeler
Acetylene black, Electrochemical impedance spectroscopy, Epoxy-substituted-polypyrrole polymer, Acetylene, Atomic force microscopy, Biocompatibility, Carbon black, Cyclic voltammetry, Electrochemical impedance spectroscopy, Esterification, Immunosensors, Indium compounds, Lighting, Polymers, Scanning electron microscopy, Tin oxides, Concentration ranges, Electrochemical immunosensors, Electrochemical signals, Esterification reactions, Indium tin oxide electrodes, Loading capacities, Optimized conditions, Selective detection, Electrochemical electrodes, acetylene, immobilized antibody, interleukin 6, polymer, polypyrrole, pyrrole derivative, African American, electrochemical analysis, electrode, genetic procedures, human, immunoassay, reproducibility, Acetylene, African Americans, Antibodies, Immobilized, Biosensing Techniques, Electrochemical Techniques, Electrodes, Humans, Immunoassay, Interleukin-6, Polymers, Pyrroles, Reproducibility of Results
Kaynak
Talanta
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
222
