Effects of triton X-100 pretreatment of lyophilized and frozen-thawed ram sperm on preimplantation embryo developmental competence

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dc.authorid0000-0002-7678-3289
dc.authorscopusid18937724600
dc.authorscopusid57190813080
dc.authorscopusid6508060684
dc.authorscopusid56099810300
dc.authorscopusid7004350993
dc.authorscopusid56779799700
dc.authorscopusid56150287300
dc.contributor.authorÜstüner, Burcu
dc.contributor.authorYağcıoğlu, Selin
dc.contributor.authorNur, Zekariya
dc.contributor.authorAlçay, Selim
dc.contributor.authorDemir, Kamber
dc.contributor.authorGökçe, Elif
dc.contributor.authorPabuccuoğlu, Serhat
dc.date.accessioned2022-05-11T14:05:13Z
dc.date.available2022-05-11T14:05:13Z
dc.date.issued2022
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü, Dölerme ve Suni Tohumlama Ana Bilim Dalı
dc.description.abstractIn this study, it was aimed to determine the effect of destruction of lyophilized and frozen-thawed ram sperm plasma and acrosomal membrane on development of embryos produced by intracytoplasmic sperm injection (ICSI). Semen samples were divided into two groups for lyophilization (L) and freezing (F). For the removal of the plasma membrane, L and F groups were incubated with Triton X-100 (LTX-100 and FTX-100, respectively). Integrities of the plasma membrane, acrosome and chromatin structure were evaluated. Oocytes were injected with these sperm groups. Although no plasma membrane and acrosome integrities of the L (0.0%) group were detected, the plasma membrane integrity of the F group (69.4%) was significantly higher than the FTX-100 group (23.6%) (p < 0.05). The acrosome integrity of the FTX-100 group (3.80%) was significantly lower than the F group (55.6%) (p < 0.05). The chromatin integrities of L and F groups were higher than the Triton X-100 treated groups (p < 0.05). ICSIs with L, LTX-100, F and FTX-100 sperm were produced similar cleavage and blastocyst rates. In conclusion, data presented here confirm that ram spermatozoa can effectively be lyophilized and injected into oocytes for initiation of embryonic development and Triton X-100 pretreatment is not necessary while using lyophilized and frozen semen.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBTAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113O593]
dc.description.sponsorshipThis study was supported financially by the Scientific and Technological Research Council of Turkey (TUBTAK; project number: 113O593).
dc.identifier.doi10.1080/10495398.2022.2041433
dc.identifier.issn1049-5398
dc.identifier.issn1532-2378
dc.identifier.pmid35200102
dc.identifier.scopus2-s2.0-85125931059
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1080/10495398.2022.2041433
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4926
dc.identifier.wosWOS:000761716600001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGökçe, Elif
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofAnimal Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIntracytoplasmic sperm injection (ICSI)
dc.subjectlyophilization
dc.subjectsheep
dc.subjectsperm plasma membrane
dc.subjecttransgenic
dc.subjectPlasma-Membrane
dc.subjectIn-Vitro
dc.subjectIntracytoplasmic Injection
dc.subjectDna Integrity
dc.subjectBlastocyst Development
dc.subjectOocytes
dc.subjectFertilization
dc.subjectSpermatozoa
dc.subjectAcrosome
dc.subjectDecondensation
dc.titleEffects of triton X-100 pretreatment of lyophilized and frozen-thawed ram sperm on preimplantation embryo developmental competence
dc.typeArticle

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