Transcriptome analysis of Rhodobacter capsulatus grown on different nitrogen sources
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
This study investigated the effect of different nitrogen sources, namely, ammonium chloride and glutamate, on photoheterotrophic metabolism of Rhodobacter capsulatus grown on acetate as the carbon source. Genes that were significantly differentially expressed according to Affymetrix microarray data were categorized into Clusters of Orthologous Groups functional categories and those in acetate assimilation, hydrogen production, and photosynthetic electron transport pathways were analyzed in detail. Genes related to hydrogen production metabolism were significantly downregulated in cultures grown on ammonium chloride when compared to those grown on glutamate. In contrast, photosynthetic electron transport and acetate assimilation pathway genes were upregulated. In detail, aceA encoding isocitrate lyase, a unique enzyme of the glyoxylate cycle and ccrA encoding the rate limiting crotonyl-CoA carboxylase/reductase enzyme of ethylmalonyl-coA pathway were significantly upregulated. Our findings indicate for the first time that R. capsulatus can operate both glyoxylate and ethylmalonyl-coA cycles for acetate assimilation.
Description
Keywords
Photoheterotrophic growth, Rhodobacter capsulatus, Purple non-sulfur bacteria, Microarray, Hydrogen metabolism, Acetate metabolism, Hydrogen-Production, Photoheterotrophic Growth, Rhodospirillum-Rubrum, Acetate Assimilation, Complex I, Metabolism, Lyase, Pathways, Fixation, Bacteria
Journal or Series
Archives of Microbiology
WoS Q Value
Q4
Scopus Q Value
Q2
Volume
201
Issue
5
