The Effect of Rosmarinic Acid on Cell Viability, Steatosis, Paraoxonase-1, and Paraoxonase-3 Protein Levels in Palmitateinduced Non-alcoholic Fatty Liver Disease Model in HepG2 Cells

Basit Öğe Kaydı
dc.contributor.authorYakşi, Esra
dc.contributor.authorÖzgün, Eray
dc.date.accessioned2023-04-28T18:43:53Z
dc.date.available2023-04-28T18:43:53Z
dc.date.issued2021
dc.departmentRektörlük, Rektörlüğe Bağlı Birimler, NKÜ Dergileri
dc.description.abstractAim: We aimed to investigate the effect of rosmarinic acid (RA) on cell viability, steatosis, paraoxonase (PON)1, and PON3 protein levels inpalmitate-induced non-alcoholic fatty liver disease (NAFLD) model in HepG2 cells.Materials and Methods: To induce an experimental steatosis model, HepG2 cells were incubated with 1 mM palmitate for 24 hours. For thetreatment, non-toxic RA concentrations were added to the cell culture medium simultaneously with the palmitate. Cell viability was evaluated by3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. To evaluate steatosis, intracellular triglyceride levels were measured andthe cells were examined microscopically with Oil-Red O staining. PON1 and PON3 protein levels were measured by Western blotting.Results: 1 mM palmitate caused a significant decrease in cell viability and a significant increase in triglyceride levels, but it did not significantlychange PON1 and PON3 protein levels. RA caused a significant increase in cell viability and a significant decrease in triglyceride levels in thepalmitate-treated cells. Similar findings with the triglyceride levels of cells were shown in microscopic examination of cells that were stained withOil-Red O. RA did not significantly change PON1 and PON3 protein levels in neither non-treated cells nor treated cells with palmitate.Conclusion: Our study showed that RA increases cell viability and decreases steatosis, but it does not change PON1 and PON3 protein levels inpalmitate-induced NAFLD model in HepG2 cells.
dc.identifier.doi10.4274/nkmj.galenos.2021.820512
dc.identifier.endpage142
dc.identifier.issn2587-0262
dc.identifier.issue2en_US
dc.identifier.startpage135
dc.identifier.trdizinid451094
dc.identifier.urihttps://doi.org10.4274/nkmj.galenos.2021.820512
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/451094
dc.identifier.urihttps://hdl.handle.net/20.500.11776/11773
dc.identifier.volume9
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofNamık Kemal Tıp Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleThe Effect of Rosmarinic Acid on Cell Viability, Steatosis, Paraoxonase-1, and Paraoxonase-3 Protein Levels in Palmitateinduced Non-alcoholic Fatty Liver Disease Model in HepG2 Cells
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
11773.pdf
Boyut:
1.13 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu
Namık Kemal Tıp Dergisi