Is Favipiravir a Potential Therapeutic Agent in the Treatment of Intervertebral Disc Degeneration by Suppressing Autophagy and Apoptosis?

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dc.authoridYILMAZ, Ibrahim/0000-0003-2003-6337
dc.authorscopusid35197435300
dc.authorscopusid57216912962
dc.authorscopusid56769801000
dc.authorscopusid56674583800
dc.authorscopusid7005912992
dc.authorscopusid56250943500
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorAkalan, Hande
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorAteş, Özkan
dc.date.accessioned2023-04-20T08:01:17Z
dc.date.available2023-04-20T08:01:17Z
dc.date.issued2022
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentYüksekokullar, Sağlık Yüksekokulu, Beslenme ve Diyetetik Bölümü
dc.description.abstractAIM: To evaluate the effects of favipiravir (FVP) on cell viability and cytotoxicity in human degenerated primary intervertebral disc (IVD) tissue cell cultures. Furthermore, the protein expressions of hypoxia-inducible factor 1 alpha (HIF-1 alpha), nuclear factor-kappa-b (NF-kappa B), and interleukin-1 beta (IL-1 beta) were also examined. MATERIAL and METHODS: Untreated cell cultures served as the control group, named group 1. Cell cultures treated with FVP served as the study group, named group 2. Pharmacomolecular analyses were performed in all groups at 0, 24, 48, and 72 hours (h). Obtained data were evaluated statistically. RESULTS: Cell proliferation was suppressed in the FVP-treated samples compared to the control group samples at 24 and 72 h, and this was statistically significant (p<0.05). Decreased or increased protein expression levels of HIF-1 alpha, NF-kappa B, and IL-1 beta in FVP-treated samples may be an indication of suppression in anabolic events as well as proliferation in IVD cultures. FVP administration showed that AF/NP cells in a culture medium may induce a strong inflammatory response to FVP. This strong inflammatory response is likely to cause slowed proliferation. It may also be a trigger for many catabolic events. NF-kappa B expression increased within the first 24 h and then decreased rapidly. Based on the data obtained, it may be suggested that the rapidly increasing NF-kB may have stimulated the expression of many antiproliferative genes. CONCLUSION: The suppression of IL-1 beta and NF-kB protein expressions in IVD cells treated with FVP is important in the treatment of IVD degeneration (IDD). If the protein expression of HIF-1 alpha could be increased along with the suppression of IL-1 beta and NF-kB, FVP would perhaps be a promising pharmacological agent in the treatment of IDD.
dc.identifier.doi10.5137/1019-5149.JTN.38252-22.3
dc.identifier.endpage687
dc.identifier.issn1019-5149
dc.identifier.issue4en_US
dc.identifier.pmid35652184
dc.identifier.scopus2-s2.0-85135031957
dc.identifier.scopusqualityQ3
dc.identifier.startpage680
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.38252-22.3
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10850
dc.identifier.volume32
dc.identifier.wosWOS:000838824600020
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAkalan, Hande
dc.institutionauthorŞirin, Duygu Yaşar
dc.language.isoen
dc.publisherTurkish Neurosurgical Soc
dc.relation.ispartofTurkish Neurosurgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectFavipiravir
dc.subjectHif-1 Alpha
dc.subjectIl-1 Beta
dc.subjectNf-Kappa B
dc.subjectNf-Kappa-B
dc.subjectNucleus Pulposus Cells
dc.subjectInflammatory Cytokines
dc.subjectSignaling Pathway
dc.subjectHif-1-Alpha
dc.subjectExpression
dc.subjectOsteoarthritis
dc.subjectInhibitor
dc.subjectCovid-19
dc.subjectHypoxia
dc.titleIs Favipiravir a Potential Therapeutic Agent in the Treatment of Intervertebral Disc Degeneration by Suppressing Autophagy and Apoptosis?
dc.typeArticle

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