Autotetraploid plant production in endemic Onobrychis elata with colchicine treatments

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dc.authorscopusid15821686200
dc.authorscopusid57225437022
dc.authorscopusid56225424300
dc.authorwosidTuna, Metin/ABA-4295-2020
dc.authorwosidAvcı, Süleyman/AAA-5525-2021
dc.contributor.authorAvcı, Süleyman
dc.contributor.authorTuna, Metin
dc.contributor.authorKaya, Mehmet Demir
dc.date.accessioned2022-05-11T14:45:52Z
dc.date.available2022-05-11T14:45:52Z
dc.date.issued2019
dc.departmentFakülteler, Ziraat Fakültesi, Tarla Bitkileri Bölümü
dc.description.abstractThis study aimed to induce autotetraploidy in endemic Onobrychis elata plants by colchicine treatment of seeds or seedlings. Colchicine was applied to O. elata directly on germinated seeds, pre-germinated seeds (root length of 3-8 mm), and apical regions (using cotton) under in vivo conditions. Out of a total of 1,210 colchicine-treated seeds that were evaluated, only 203 survived. There was an inverse relationship between the number of surviving plants and colchicine concentration and exposure time. The highest percentage of tetraploidy in surviving plants (50%) was obtained by applying 0.2% colchicine for 6 hours to pre-germinated seeds. No significant tetraploidy was achieved by colchicine application to seedlings. Flow cytometry observations indicated that DNA content varied between 0.99 and 1.06 pg in diploid plants (controls), while DNA content varied between 2.22 and 2.48 pg in tetraploid plants. It was concluded that tetraploid plants were induced successfully only in seedlings obtained from pre-germinated seeds, with their ploidy level confirmed via flow cytometry analysis.
dc.description.sponsorshipEskisehir Osmangazi UniversityEskisehir Osmangazi University [2016-1030]
dc.description.sponsorshipThe author thanks Eskisehir Osmangazi University for its financial support (Project number: 2016-1030).
dc.identifier.doi10.5073/JABFQ.2019.092.012
dc.identifier.endpage93
dc.identifier.issn1439-040X
dc.identifier.scopus2-s2.0-85068059853
dc.identifier.scopusqualityQ3
dc.identifier.startpage88
dc.identifier.urihttps://doi.org/10.5073/JABFQ.2019.092.012
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10168
dc.identifier.volume92
dc.identifier.wosWOS:000468314600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorTuna, Metin
dc.language.isoen
dc.publisherDruckerei Liddy Halm
dc.relation.ispartofJournal of Applied Botany and Food Quality
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectOnobrychis
dc.subjectcolchicine
dc.subjectflow cytometry
dc.subjectpolyploidy
dc.subjectInduced Tetraploids
dc.subjectInduction
dc.titleAutotetraploid plant production in endemic Onobrychis elata with colchicine treatments
dc.typeArticle

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