Are chondrocytes damaged when rheumatologic inflammation is suppressed?

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dc.authorid0000-0003-2003-6337
dc.authorscopusid57191926529
dc.authorscopusid56258081800
dc.authorscopusid35197435300
dc.authorscopusid56769801000
dc.authorscopusid23102238400
dc.authorscopusid56002899200
dc.authorwosidMahirogullari, Mahir/AAA-4742-2020
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.contributor.authorYıldırım Güzelant, Aliye
dc.contributor.authorİşyar, Mehmet
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorÇakmak, Selami
dc.contributor.authorMahiroğulları, Mahir
dc.date.accessioned2022-05-11T14:12:16Z
dc.date.available2022-05-11T14:12:16Z
dc.date.issued2017
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Fizik Tedavi ve Rehabilitasyon Ana Bilim Dalı
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractAim: The use of biological agents (BAs) for treating diseases such as rheumatoid arthritis (RA), spondyloarthropathy, and systemic lupus erythematosus to reduce inflammation has been fruitful. Especially as part of the increasing number of studies on the intra-articular application of BAs, the effects of BAs on cartilage have been widely investigated. In the present study, the effects of rituximab, abatacept, and adalimumab, all approved antirheumatic agents, on human primary chondrocytes were investigated comparatively and on the molecular level through viability, proliferation, and toxicity analyses. Materials and methods: Osteochondral tissues from the distal femur and proximal tibia were resected during total knee arthroplasty from patients (n=3) with confirmed gonarthrosis in whom all medical or conservative treatments had failed. Standard human primary chondrocyte cell culturing was carried out. Immunophenotyping was performed on the cells that adhered to the flask, and their chondrotoxicity was observed using a flow cytometry device. Images of the cells showing chondrotoxicity were analyzed using invert and environmental scanning microscopes, and microimages were obtained. The MTT-enzyme linked immunosorbent assay was performed to observe the toxic effects of BAs on the proliferation of chondrocytes at 24 and 48h. The results were analyzed using the number of cells and proliferation; statistical comparisons among the groups were carried out using one-way ANOVA. The alpha significance level was set at <0.01. Results: These pharmaceutical agents were chondrotoxic, especially on viability and proliferation (p=0.0000). Conclusion: BAs are generally used during active inflammation, and following the management of inflammation, their dosage should be determined taking into consideration their cellular-level toxic effects on chondrocytes.
dc.identifier.doi10.3109/01480545.2016.1166249
dc.identifier.endpage23
dc.identifier.issn0148-0545
dc.identifier.issn1525-6014
dc.identifier.issue1en_US
dc.identifier.pmid27079996
dc.identifier.scopus2-s2.0-84963631961
dc.identifier.scopusqualityQ2
dc.identifier.startpage13
dc.identifier.urihttps://doi.org/10.3109/01480545.2016.1166249
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5472
dc.identifier.volume40
dc.identifier.wosWOS:000395010200003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorYıldırım Güzelant, Aliye
dc.institutionauthorŞirin, Duygu Yaşar
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofDrug and Chemical Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAbatacept
dc.subjectadalimumab
dc.subjectcell culture
dc.subjectchondrotoxicity
dc.subjectrituximab
dc.subjectArthritis
dc.subjectAdalimumab
dc.subjectTherapies
dc.subjectCartilage
dc.subjectAbatacept
dc.subjectDisease
dc.subjectAgents
dc.subjectCells
dc.titleAre chondrocytes damaged when rheumatologic inflammation is suppressed?
dc.typeArticle

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