Construction and Comparison of Trametes versicolor Laccase Biosensors Capable of Detecting Xenobiotics
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Date
2010
Journal Title
Journal ISSN
Volume Title
Publisher
Informa Healthcare
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Amperometric biosensors using laccase from Trametes versicolor as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). Laccase enzyme was immobilized by gelatin and glutaraldehyde onto a Clark oxygen probe and screen printed electrodes (SPEs). Amperometric and chronoamperometric measurements were carried out with the biosensors. First, the effect of laccase activity on the biosensor performances was investigated for both biosensors, and then optimum pH and temperature and also thermal stability of the biosensors were tested. In addition, the detection ranges of some phenolic compounds were obtained by the help of calibration graphs of them. In repeatability studies, variation coefficients and standard deviations for both biosensors were also calculated by the studies done for this purposes. Finally, the biosensors were applied to the determination of 2,4-D in a real herbicide sample.
Description
Keywords
laccase biosensors, screen printed electrodes, 2,4-D, xenobiotic, herbicides, Trametes versicolor laccase, Chlorophenoxy Acid Herbicides, 2,4-Dichlorophenoxyacetic Acid, Mediator Systems, 2,4-D, Water, Derivatization, Detoxification, Enzymes, Elisa
Journal or Series
Artificial Cells Blood Substitutes and Biotechnology
WoS Q Value
Q4
Scopus Q Value
N/A
Volume
38
Issue
4
