Construction and Comparison of Trametes versicolor Laccase Biosensors Capable of Detecting Xenobiotics

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Küçük Resim

Tarih

2010

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Informa Healthcare

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Amperometric biosensors using laccase from Trametes versicolor as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). Laccase enzyme was immobilized by gelatin and glutaraldehyde onto a Clark oxygen probe and screen printed electrodes (SPEs). Amperometric and chronoamperometric measurements were carried out with the biosensors. First, the effect of laccase activity on the biosensor performances was investigated for both biosensors, and then optimum pH and temperature and also thermal stability of the biosensors were tested. In addition, the detection ranges of some phenolic compounds were obtained by the help of calibration graphs of them. In repeatability studies, variation coefficients and standard deviations for both biosensors were also calculated by the studies done for this purposes. Finally, the biosensors were applied to the determination of 2,4-D in a real herbicide sample.

Açıklama

Anahtar Kelimeler

laccase biosensors, screen printed electrodes, 2,4-D, xenobiotic, herbicides, Trametes versicolor laccase, Chlorophenoxy Acid Herbicides, 2,4-Dichlorophenoxyacetic Acid, Mediator Systems, 2,4-D, Water, Derivatization, Detoxification, Enzymes, Elisa

Kaynak

Artificial Cells Blood Substitutes and Biotechnology

WoS Q Değeri

Q4

Scopus Q Değeri

N/A

Cilt

38

Sayı

4

Künye