Construction and Comparison of Trametes versicolor Laccase Biosensors Capable of Detecting Xenobiotics
Yükleniyor...
Dosyalar
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Informa Healthcare
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Amperometric biosensors using laccase from Trametes versicolor as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-D). Laccase enzyme was immobilized by gelatin and glutaraldehyde onto a Clark oxygen probe and screen printed electrodes (SPEs). Amperometric and chronoamperometric measurements were carried out with the biosensors. First, the effect of laccase activity on the biosensor performances was investigated for both biosensors, and then optimum pH and temperature and also thermal stability of the biosensors were tested. In addition, the detection ranges of some phenolic compounds were obtained by the help of calibration graphs of them. In repeatability studies, variation coefficients and standard deviations for both biosensors were also calculated by the studies done for this purposes. Finally, the biosensors were applied to the determination of 2,4-D in a real herbicide sample.
Açıklama
Anahtar Kelimeler
laccase biosensors, screen printed electrodes, 2,4-D, xenobiotic, herbicides, Trametes versicolor laccase, Chlorophenoxy Acid Herbicides, 2,4-Dichlorophenoxyacetic Acid, Mediator Systems, 2,4-D, Water, Derivatization, Detoxification, Enzymes, Elisa
Kaynak
Artificial Cells Blood Substitutes and Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
38
Sayı
4