Protective and Therapeutic Effects of Bovine Amniotic Fluids Collected in Different Trimesters on the Epidural Fibrosis After Experimental Laminectomy in Rats

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dc.contributor.authorYurtal, Ziya
dc.contributor.authorKulualp, Kadri
dc.contributor.authorÖzkan, Hüseyin
dc.contributor.authorMıcılı, Serap Cilaker
dc.contributor.authorDoğan, Halef
dc.contributor.authorŞişman, Ali Riza
dc.contributor.authorErsoy, Nevin
dc.date.accessioned2023-05-06T17:23:36Z
dc.date.available2023-05-06T17:23:36Z
dc.date.issued2023
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü, Doğum ve Jinekoloji Ana Bilim Dalı
dc.description.abstractBACKGROUND: The aim of this study was to investigate the protective and therapeutic effects of bovine amniotic fluid (BAF) on the inhibition of epidural fibrosis (EF) after experimental laminectomy. -METHODS: Forty female Sprague Dawley rats were used. The amniotic fluids were collected from each trimester of a pregnant cow. The rats were divided into 5 groups. Whereas no laminectomy was applied to the con-trol group, animals in the sham group underwent lam-inectomy. Laminectomy was performed in the animals in other groups and the operation area was closed by drip-ping 1 mL of BAF collected in 3 trimesters of pregnancy. Animals were killed 28 days after the operation.RESULTS: Compared with control, VEGF gene expression levels were downregulated approximately 5-fold in BAF-2. Whereas IL-6 was upregulated approximately 8-fold in the sham, it was downregulated 5-fold and 3-fold in BAF-1 and BAF-2, respectively. There was downregulation in BAF-2 and BAF-3 in terms of CD105 gene expression levels. TGFb1 was upregulated approximately 2-fold in the sham group and downregulated in BAF-1 and BAF-2. Although histopathologic alterations including EF grade and fibro-blast cell density were found to increase in the sham group, all BAF treatment decreased those of alterations. The highest CD105 immunoreactivity was detected in the sham group. All BAF treatment markedly aggravated fibrosis via decreasing CD105 immunoreactivity. In terms of grading parameters, almost the closest grades to the con-trol were determined in the BAF-2. BAF collected in the second trimester is most effective in healing of scar tissue and preventing fibrosis via decreasing microvessel and fibroblast densities.CONCLUSIONS: The results indicate that BAF may be used as a potential protective agent to prevent EF.
dc.description.sponsorshipDokuz Eylul University Scientific Research Project Foundation [2021, KB.SAG.037]
dc.description.sponsorshipConflict of interest statement: This work was financially supported by Dokuz Eylul University Scientific Research Project Foundation grant number 2021.KB.SAG.037.
dc.identifier.doi10.1016/j.wneu.2022.12.092
dc.identifier.endpageE730
dc.identifier.issn1878-8750
dc.identifier.issn1878-8769
dc.identifier.pmid36608801
dc.identifier.scopus2-s2.0-85147576323
dc.identifier.scopusqualityQ2
dc.identifier.startpageE722
dc.identifier.urihttps://doi.org/10.1016/j.wneu.2022.12.092
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12187
dc.identifier.volume171
dc.identifier.wosWOS:000949441300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDoğan, Halef
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.ispartofWorld Neurosurgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBovine amniotic fluids
dc.subjectEpidural fibrosis
dc.subjectInflammation
dc.subjectLaminectomy
dc.subjectHyaluronic-Acid
dc.subjectEndoglin Cd105
dc.subjectMembrane
dc.subjectExpression
dc.subjectPrevention
dc.subjectAngiogenesis
dc.subjectFibroblasts
dc.subjectCarcinoma
dc.subjectAdhesion
dc.subjectEfficacy
dc.titleProtective and Therapeutic Effects of Bovine Amniotic Fluids Collected in Different Trimesters on the Epidural Fibrosis After Experimental Laminectomy in Rats
dc.typeArticle

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