A novel immunosensor based on fullerene C60 for electrochemical analysis of heat shock protein 70

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dc.authorscopusid56106371600
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorDemirbakan, Burcak
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:30:53Z
dc.date.available2022-05-11T14:30:53Z
dc.date.issued2016
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractThis study illustrates the development of the biosensor for the determination of the heat shock protein 70 (HSP70). For this purpose, firstly the biosensor based on the glassy carbon electrode (GCE) was modified with fullerene C-60. The anti-HSP70 was bound on fullerene C-60 layer by using the EDC/NHS couple. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods were applied to characterize the immobilization of anti-HSP70 process and to determine the HSP70. In order to clarify the binding characterization of HSP70 and anti-HSP70, a single frequency impedance method was utilized, whereas the scanning electron microscopy was used to identify the surface morphology and Kramers-Kronig transform was implemented on impedance data. The biosensor exhibited good repeatability and reproducibility. The linear detection range of the developed biosensor was of 0.8 pg-12.8 pg/mL. Real human blood serum samples were analyzed by the biosensor and the results were also validated by using a reference method based on ELISA. (C) 2016 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipThis work was funded by TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) whose assistance is greatly acknowledged.
dc.identifier.doi10.1016/j.jelechem.2016.11.020
dc.identifier.endpage207
dc.identifier.issn1572-6657
dc.identifier.issn1873-2569
dc.identifier.scopus2-s2.0-85002373823
dc.identifier.scopusqualityQ1
dc.identifier.startpage201
dc.identifier.urihttps://doi.org/10.1016/j.jelechem.2016.11.020
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7215
dc.identifier.volume783
dc.identifier.wosWOS:000390627500028
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorDemirbakan, Burcak
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofJournal of Electroanalytical Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectFullerene C-60
dc.subjectHeat shock protein (HSP70)
dc.subjectBiosensor
dc.subjectElectrochemical impedance spectroscopy (EIS)
dc.subjectCancer biomarkers
dc.subjectHeat-Shock Proteins
dc.subjectHsp70
dc.subjectC-60
dc.subjectInhibition
dc.subjectElectrodes
dc.subjectBiosensors
dc.subjectBiomarker
dc.subjectSystems
dc.subjectDeath
dc.subjectCells
dc.titleA novel immunosensor based on fullerene C60 for electrochemical analysis of heat shock protein 70
dc.typeArticle

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