Characterization of Infectious Laryngotracheitis Virus Isolates from Turkey by Molecular and Sequence Analysis

Basit Öğe Kaydı
dc.authorid0000-0003-2116-105X
dc.authorid0000-0003-2822-560X
dc.authorid0000-0002-5811-2588
dc.authorscopusid24597452800
dc.authorscopusid57103691500
dc.authorscopusid57217233918
dc.authorscopusid8896086300
dc.authorscopusid7003605484
dc.authorwosidAbayli, Hasan/W-1858-2018
dc.authorwosidtonbak, sukru/W-2199-2018
dc.authorwosidErtan, Asli/AAE-6201-2021
dc.authorwosidBulut, Hakan/ABA-1433-2020
dc.contributor.authorCan-Sahna, Kezban
dc.contributor.authorAbaylı, Hasan
dc.contributor.authorÖzbek, Remziye
dc.contributor.authorTonbak, Sukm
dc.contributor.authorBulut, Hakan
dc.date.accessioned2022-05-11T14:42:22Z
dc.date.available2022-05-11T14:42:22Z
dc.date.issued2020
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Viroloji Ana Bilim Dalı
dc.description.abstractInfectious laryngotracheitis (ILT) is an economically important respiratory disease affecting the poultry industry worldwide. The aim of this study was to characterize the Turkish ILT virus (ILTV) isolates by sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). ILTV isolates obtained from laying hens in Turkey in 2003 and 2018 were used in this study. The isolates were analyzed for ICP4, gG, gE and TK gene regions by PCR. The amplification products were used in RFLP analysis to determine the differences among the isolates. Sequence analyses of TK and ICP4 regions were carried out and a phylogenetic tree was formed by using the Maximum Likelihood method. Nucleotide identity values were then calculated among five isolates and other strains/isolates in Genbank. In addition, about 200 amino acid sequences of the start and end regions of the ICP4 gene were compared to other strains in Genbank. PCR-RFLP analysis indicated that Turkish ILTV isolates were low-virulent. In general, the nucleotide sequence similarities of the TK and ICP4 gene regions among Turkish isolates and others was more than 95% (lower in some Egyptian and Bangladeshi strains, 41 and 45% respectively); in the amino acid sequence, it was close to 100%. As a result, PCR-RFLP results were similar in many gene regions. However, evolutionary analysis of ICP4 and TK gene regions did not yield reliable results based on geographic distribution or pathogenicity levels. For this purpose, different methods, such as Bayesian analysis or the involvement of samples from different gene regions can yield more reliable results, just like whole-genome sequences. (C) 2020 PVJ. All rights reserved
dc.description.sponsorshipScientific Research Projects Coordination Unit of Firat UniversityFirat University [VF18.07]
dc.description.sponsorshipThis work was supported by the project number VF18.07 by the Scientific Research Projects Coordination Unit of Firat University.
dc.identifier.doi10.29261/pakvetj/2020.051
dc.identifier.endpage342
dc.identifier.issn0253-8318
dc.identifier.issn2074-7764
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85090277269
dc.identifier.scopusqualityQ2
dc.identifier.startpage337
dc.identifier.urihttps://doi.org/10.29261/pakvetj/2020.051
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9347
dc.identifier.volume40
dc.identifier.wosWOS:000555954700011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorBulut, Hakan
dc.language.isoen
dc.publisherUniv Agriculture, Fac Veterinary Science
dc.relation.ispartofPakistan Veterinary Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectILTV
dc.subjectPCR-RFLP
dc.subjectsequence analysis
dc.subjectTurkey
dc.subjectFragment-Length-Polymorphism
dc.subjectPolymerase-Chain-Reaction
dc.subjectVaccine Strains
dc.subjectField
dc.subjectDifferentiation
dc.subjectStrategies
dc.subjectRegions
dc.subjectIltv
dc.titleCharacterization of Infectious Laryngotracheitis Virus Isolates from Turkey by Molecular and Sequence Analysis
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
9347.pdf
Boyut:
387.56 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Veteriner Fakültesi Koleksiyonu