The electrostatic immobilization of the horseradish peroxidase on poly-L-histidine modified multiwall carbon nanotube-ionic liquid composite glassy carbon electrode for the amperometric determination of hydrogen peroxide

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dc.contributor.authorUlukan, Elif
dc.contributor.authoroeztuerk, Funda
dc.date.accessioned2024-10-29T17:58:18Z
dc.date.available2024-10-29T17:58:18Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractIn this study, an amperometric biosensor for hydrogen peroxide quantification was prepared by electrostatic immobilization of horseradish peroxidase on a multi-walled carbon nanotube, ionic liquid, and poly-L-histidine modified glassy carbon electrode. The immobilization of the horseradish peroxidase on the electrode surface was achieved by positive charges of poly-L-histidine. The surface morphology was analyzed by scanning electron microscopy, cyclic voltammetry, and impedance spectroscopy. The electrode composition, buffer pH, and operating potential were all optimized in order to achieve the best analytical performance. The modified electrode, which has been fabricated at its optimum composition, reveals two different linear working ranges, namely 2.0 x 10(-8) M to 9.1 x 10(-7) M and 1.8 x 10(-6) M to 1.8 x 10(-4) M, which are at the determined optimum operating conditions. The detection limit of the proposed method is of 2.9 x 10(-9) M. The analytical applicability of the amperometric determination of hydrogen peroxide concerning the developed biosensor has been evaluated by quantifying hydrogen peroxide in a commercial oxygenated water sample.{GRAPHICAL ABSTRACT}
dc.description.sponsorshipTekirdag Namik Kemal University Scientific Research Projects Coordination Unit [NKUBAP.01.GA.19.202]
dc.description.sponsorshipThis study was supported by Tekirdag Namik Kemal University Scientific Research Projects Coordination Unit (Project No: NKUBAP.01.GA.19.202).
dc.identifier.doi10.1007/s00706-023-03071-6
dc.identifier.endpage594
dc.identifier.issn0026-9247
dc.identifier.issn1434-4475
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85158139215
dc.identifier.scopusqualityQ3
dc.identifier.startpage585
dc.identifier.urihttps://doi.org/10.1007/s00706-023-03071-6
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14209
dc.identifier.volume154
dc.identifier.wosWOS:000981846900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.ispartofMonatshefte Fur Chemie
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiosensors
dc.subjectElectrochemistry
dc.subjectEnzymes
dc.subjectHydrogen peroxide
dc.subjectPoly-L-histidine
dc.titleThe electrostatic immobilization of the horseradish peroxidase on poly-L-histidine modified multiwall carbon nanotube-ionic liquid composite glassy carbon electrode for the amperometric determination of hydrogen peroxide
dc.typeArticle

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