The electrostatic immobilization of the horseradish peroxidase on poly-L-histidine modified multiwall carbon nanotube-ionic liquid composite glassy carbon electrode for the amperometric determination of hydrogen peroxide

Küçük Resim Yok

Tarih

2023

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Springer Wien

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

In this study, an amperometric biosensor for hydrogen peroxide quantification was prepared by electrostatic immobilization of horseradish peroxidase on a multi-walled carbon nanotube, ionic liquid, and poly-L-histidine modified glassy carbon electrode. The immobilization of the horseradish peroxidase on the electrode surface was achieved by positive charges of poly-L-histidine. The surface morphology was analyzed by scanning electron microscopy, cyclic voltammetry, and impedance spectroscopy. The electrode composition, buffer pH, and operating potential were all optimized in order to achieve the best analytical performance. The modified electrode, which has been fabricated at its optimum composition, reveals two different linear working ranges, namely 2.0 x 10(-8) M to 9.1 x 10(-7) M and 1.8 x 10(-6) M to 1.8 x 10(-4) M, which are at the determined optimum operating conditions. The detection limit of the proposed method is of 2.9 x 10(-9) M. The analytical applicability of the amperometric determination of hydrogen peroxide concerning the developed biosensor has been evaluated by quantifying hydrogen peroxide in a commercial oxygenated water sample.{GRAPHICAL ABSTRACT}

Açıklama

Anahtar Kelimeler

Biosensors, Electrochemistry, Enzymes, Hydrogen peroxide, Poly-L-histidine

Kaynak

Monatshefte Fur Chemie

WoS Q Değeri

Q3

Scopus Q Değeri

Q3

Cilt

154

Sayı

6

Künye