Development of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles

Basit Öğe Kaydı
dc.authorid0000-0002-2874-4998
dc.authorid0000-0002-8102-5992
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidKaragozlu, Cem/A-5430-2016
dc.authorwosidALTUĞ, Çağrı/AAG-4987-2021
dc.authorwosidMENGÜLLÜOĞLU, UMUT/AAG-7084-2021
dc.authorwosidDİNÇKAYA, ERHAN/AAG-7096-2021
dc.contributor.authorDinçkaya, Erhan
dc.contributor.authorKinik, Ozer
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.contributor.authorAltuğ, Çağrı
dc.contributor.authorAkkoca, Aylin
dc.date.accessioned2022-05-11T14:29:44Z
dc.date.available2022-05-11T14:29:44Z
dc.date.issued2011
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractThe present work describes the construction and application of a new DNA biosensor for detection of aflatoxin M1. In order to immobilize a thiol-modified single stranded DNA (ss-HSDNA) probe that specifically bound aflatoxin M1, a self-assembled monolayer of cysteamine and gold nanoparticles on the SAM were prepared on gold electrodes, layer-by-layer. The assembly processes of cysteamine, gold nanoparticles, and ss-HSDNA were monitored with the help of electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. K-3[Fe(CN)(6)]/K-4[Fe(CN)(6)] solution was used as a redox probe for electrochemical measurements. The biosensor provided a linear response to aflatoxin M1 over the concentration range of 1-14 ng/mL with a standard deviation of +/- 0.36 ng/mL Finally, the biosensor was applied to a series of real milk samples. (C) 2011 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBiTAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [106T 367]; EBiLTEM (Ege University of Research and Application Center of Science and Technology)Ege University [2007 BiL 013]
dc.description.sponsorshipThis study was supported by TUBiTAK (The Scientific and Technological Research Council of Turkey, Project number: 106T 367) and EBiLTEM (Ege University of Research and Application Center of Science and Technology, Project number: 2007 BiL 013).
dc.identifier.doi10.1016/j.bios.2011.02.038
dc.identifier.endpage3811
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.issue9en_US
dc.identifier.pmid21420290
dc.identifier.startpage3806
dc.identifier.urihttps://doi.org/10.1016/j.bios.2011.02.038
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7116
dc.identifier.volume26
dc.identifier.wosWOS:000291178200013
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevier Advanced Technology
dc.relation.ispartofBiosensors & Bioelectronics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAflatoxin M1
dc.subjectElectrochemical impedance spectroscopy
dc.subjectBiosensor
dc.subjectGold nanoparticles
dc.subjectSelf-assembled monolayers
dc.subjectMycotoxins
dc.subjectElectrochemical Immunosensor
dc.subjectMilk-Products
dc.subjectM-1
dc.subjectImmunoassay
dc.subjectMycotoxins
dc.subjectSamples
dc.subjectFungi
dc.subjectElisa
dc.subjectM(1)
dc.subjectHplc
dc.titleDevelopment of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
7116.pdf
Boyut:
406.45 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Fen Edebiyat Fakültesi Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu