Development of an impedimetric aflatoxin M1 biosensor based on a DNA probe and gold nanoparticles
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Date
2011
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Advanced Technology
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
The present work describes the construction and application of a new DNA biosensor for detection of aflatoxin M1. In order to immobilize a thiol-modified single stranded DNA (ss-HSDNA) probe that specifically bound aflatoxin M1, a self-assembled monolayer of cysteamine and gold nanoparticles on the SAM were prepared on gold electrodes, layer-by-layer. The assembly processes of cysteamine, gold nanoparticles, and ss-HSDNA were monitored with the help of electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. K-3[Fe(CN)(6)]/K-4[Fe(CN)(6)] solution was used as a redox probe for electrochemical measurements. The biosensor provided a linear response to aflatoxin M1 over the concentration range of 1-14 ng/mL with a standard deviation of +/- 0.36 ng/mL Finally, the biosensor was applied to a series of real milk samples. (C) 2011 Elsevier B.V. All rights reserved.
Description
Keywords
Aflatoxin M1, Electrochemical impedance spectroscopy, Biosensor, Gold nanoparticles, Self-assembled monolayers, Mycotoxins, Electrochemical Immunosensor, Milk-Products, M-1, Immunoassay, Mycotoxins, Samples, Fungi, Elisa, M(1), Hplc
Journal or Series
Biosensors & Bioelectronics
WoS Q Value
Q1
Scopus Q Value
Volume
26
Issue
9
