Identification and Genetic Characterization of Pseudomonas syringae pv. syringae from Sweet Cherry in Turkey

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dc.authoridBaysal-Gurel, Fulya/0000-0001-9920-9880
dc.authoridAvin, Farhat Ahmadi/0000-0002-4323-1058
dc.authorscopusid56576393600
dc.authorscopusid54966780500
dc.authorscopusid55948751100
dc.authorscopusid25637052100
dc.authorwosidAvin, Farhat Ahmadi/D-8062-2015
dc.contributor.authorÖksel, Cansu
dc.contributor.authorAvin, Farhat A.
dc.contributor.authorMırık, Mustafa
dc.contributor.authorBaysal-Gürel, Fulya
dc.date.accessioned2023-04-20T08:01:15Z
dc.date.available2023-04-20T08:01:15Z
dc.date.issued2022
dc.departmentFakülteler, Ziraat Fakültesi, Bitki Koruma Bölümü
dc.description.abstractPseudomonas syringae pv. syringae, which causes bacterial canker, is the most polyphagous bacterium in the P. syringae complex because of its broad host range. This pathogen is considered the major bacterial disease in cherry orchards. In this study, several samples were collected from infected sweet cherry (Prunus avium L.) trees in different locations of the Marmara region in Turkey between 2016 and 2018. Sixty-three isolates were identified as P. syringae pv. syringae by pathogenicity, LOPAT, GATTa, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry tests. Total genomic DNA was extracted to confirm identity, followed by PCR amplification of syrB and cfl genes. Out of 63 isolates, 12 were randomly selected for repetitive element sequence-based PCR and multilocus sequence typing analyses to gain insight into the relationships of those isolates. The cluster analysis of enterobacterial repetitive intergenic consensus-, repetitive extragenic palindromic-, and BOX-A1R-based repetitive extragenic-palindromic-PCR techniques could classify the isolates into two distinct clusters. Phylogenetic analysis was carried out to obtain the relation between isolates and the location. The multilocus sequencing typing analysis of gyrB, rpoDp, rpoDs, and gltA genes allowed a clear allocation of the isolates into two separate main clusters. The relationships among the isolates were also evaluated by constructing a genealogical median-joining network (MJN). The isolates from six locations produced 11 haplotypes that were illustrated in the MJN. The results of this study proved that location could not be an indicator for showing the genetic diversity of P. syringae pv. syringae from cherry orchards. As the genetic variability of Pseudomonads has been demonstrated, this study also showed high diversity among different isolates even within the populations. While more research is recommended, the results of this study contributed to a better understanding of the evolutionary progress of P. syringae pv. syringae and the genetic diversity of sweet cherry isolates.
dc.description.sponsorshipNational Institute of Food and Agriculture [TENX-S-1083]; Scientific and Technological Research Council of Turkey; TUBITAK [2214-A 1059B141801417]
dc.description.sponsorshipThis work was supported by the National Institute of Food and Agriculture under award nos. TENX-1926-CCOCP and TENX-S-1083, and the Scientific and Technological Research Council of Turkey under award no. TUBITAK 2214-A 1059B141801417.
dc.identifier.doi10.1094/PDIS-10-21-2241-RE
dc.identifier.endpage1261
dc.identifier.issn0191-2917
dc.identifier.issn1943-7692
dc.identifier.issue4en_US
dc.identifier.pmid34818912
dc.identifier.scopus2-s2.0-85124416092
dc.identifier.scopusqualityQ1
dc.identifier.startpage1253
dc.identifier.urihttps://doi.org/10.1094/PDIS-10-21-2241-RE
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10829
dc.identifier.volume106
dc.identifier.wosWOS:000784156500021
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖksel, Cansu
dc.institutionauthorMırık, Mustafa
dc.language.isoen
dc.publisherAmer Phytopathological Soc
dc.relation.ispartofPlant Disease
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBacterial Canker
dc.subjectMultilocus Sequence Typing
dc.subjectPhylogenetic Analysis
dc.subjectPrunus Avium
dc.subjectRepetitive Element Sequence-Based Pcr
dc.subjectBacterial Canker
dc.subjectStone Fruits
dc.subjectWild Cherry
dc.subjectSour Cherry
dc.subjectPathovars
dc.subjectStrains
dc.subjectDiversity
dc.subjectCultivars
dc.subjectTrees
dc.subjectEvolution
dc.titleIdentification and Genetic Characterization of Pseudomonas syringae pv. syringae from Sweet Cherry in Turkey
dc.typeArticle

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