Label-free, ITO-based immunosensor for the detection of a cancer biomarker: Receptor for Activated C Kinase 1

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dc.authorscopusid56465916100
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorBahadır, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:12:19Z
dc.date.available2022-05-11T14:12:19Z
dc.date.issued2016
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractA new, quite sensitive disposable immunosensor, based on the anti-RACK1 antibody, was developed for the determination of Receptor for Activated C Kinase 1 (RACK1) for the first time. Moreover, indium tin oxide (ITO) flexible sheets were modified by using aldehyde ended silane 11-(triethoxysilyl) undecanal (TESU) self-assembled monolayers (SAMs) for immobilizing the anti-RACK1 antibody via covalent bonds. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and square wave voltammetry (SWV) methods were used for characterizing the immobilization steps and for determining the RACK1 concentration. To obtain a successful immunosensor, experimental factors were optimized. This quite sensitive biosensor was able to detect concentrations as low as fg mL(-1). The detection principle was based on the change in charge transfer resistance and the Nyquist plot diameter and these changes were monitored by using electrochemical impedance spectroscopy (EIS). This impedimetric immunosensor had perfect repeatability and reproducibility. We proved that the new silane agent, TESU, performs well in biosensor applications. The feasibility of the fabricated immunosensor was tested by detecting RACK1 in artificial and real human serum samples.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipThe authors acknowledge financial support from The Scientific and Technological Research Council of Turkey (TUBITAK) (Project Number: 113 Z 678).
dc.identifier.doi10.1039/c6an00694a
dc.identifier.endpage5626
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.issue19en_US
dc.identifier.pmid27454557
dc.identifier.scopus2-s2.0-84988624715
dc.identifier.scopusqualityQ1
dc.identifier.startpage5618
dc.identifier.urihttps://doi.org/10.1039/c6an00694a
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5501
dc.identifier.volume141
dc.identifier.wosWOS:000384309800017
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBahadır, Elif Burcu
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofAnalyst
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIndium-Tin Oxide
dc.subjectSelf-Assembled Monolayer
dc.subjectGold Nanoparticles
dc.subjectGlucose-Oxidase
dc.subjectElectrode
dc.subjectRack1
dc.subjectImmobilization
dc.subjectDeposition
dc.subjectPredictor
dc.subjectComposite
dc.titleLabel-free, ITO-based immunosensor for the detection of a cancer biomarker: Receptor for Activated C Kinase 1
dc.typeArticle

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