Beta-galactosidase Determination by an Electrochemical Biosensor Mediated with Ferrocene

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dc.authorscopusid6506764918
dc.authorscopusid6603789064
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidDİNÇKAYA, ERHAN/AAG-7096-2021
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.contributor.authorDinçkaya, Erhan
dc.date.accessioned2022-05-11T14:29:44Z
dc.date.available2022-05-11T14:29:44Z
dc.date.issued2011
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractIn this paper, a new viewpoint on the activity determination of beta-galactosidase is reported. Glucose oxidase was directly immobilized on a glassy carbon electrode and mediated by ferrocene. The biosensor's performance was based on mediated electron transfer by ferrocene, which reduced via glucose oxidase reaction. In this reaction, substrate of glucose oxidase, glucose was provided by the activity of beta-galactosidase in the sample. The parameters of the fabrication process for the electrode were optimized. Experimental conditions influencing the biosensor performance, such as pH, ferrocene and lactose concentrations, and temperature, were investigated and assessed. Finally, the biosensor was successfully applied to determination of beta-galactosidase activity of artificial intestinal juice.
dc.description.sponsorshipEge UniversityEge University; Scientific Research Foundation [2004 FEN 002]; Scientific and Technical Research Council of Turkiye (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)
dc.description.sponsorshipThe authors wish to thank for financial support Ege University, Scientific Research Foundation (Grant No. 2004 FEN 002), and The Scientific and Technical Research Council of Turkiye (TUBITAK).
dc.identifier.doi10.3109/10731199.2011.559644
dc.identifier.endpage273
dc.identifier.issn1073-1199
dc.identifier.issue5en_US
dc.identifier.pmid21338333
dc.identifier.scopus2-s2.0-80052692576
dc.identifier.scopusqualityN/A
dc.identifier.startpage267
dc.identifier.urihttps://doi.org/10.3109/10731199.2011.559644
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7113
dc.identifier.volume39
dc.identifier.wosWOS:000294740300001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherInforma Healthcare
dc.relation.ispartofArtificial Cells Blood Substitutes and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbeta-galactosidase
dc.subjectbiosensor
dc.subjectglucose oxidase
dc.subjectferrocene
dc.subjectenzyme activity determination
dc.subjectPerformance Liquid-Chromatography
dc.subjectLysosomal Storage Disorders
dc.subjectEscherichia-Coli
dc.subjectIn-Vivo
dc.subjectGm1
dc.subjectDegradation
dc.subjectMicroassay
dc.subjectProtein
dc.subjectWater
dc.titleBeta-galactosidase Determination by an Electrochemical Biosensor Mediated with Ferrocene
dc.typeArticle

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