Covalent Immobilization of Urease on Poly(Pyrrole-3-carboxylic Acid): Electrochemical Impedance and Mott Schottky Study

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dc.authorid0000-0002-1806-0330
dc.authorid0000-0001-7513-1740
dc.authorscopusid54941444900
dc.authorscopusid9735216100
dc.authorscopusid35548936600
dc.authorwosidAtes, Murat/G-3798-2012
dc.authorwosidSarac, A.Sezai/N-1628-2014
dc.contributor.authorKarazehir, Tolga
dc.contributor.authorAteş, Murat
dc.contributor.authorSaraç, A. Sezai
dc.date.accessioned2022-05-11T14:30:54Z
dc.date.available2022-05-11T14:30:54Z
dc.date.issued2016
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractPyrrole-3-carboxylic acid was electropolymerized on a glassy carbon electrode in 0.1 M NaClO4/ACN, Et4NBF4/ACN using cyclic voltammetry. Urease (Urs) was immobilized onto PPCA modified GCE via covalent binding, exploiting EDC/NHS chemistry. Electrochemical impedance spectroscopy was used to investigate the interfacial behavior of Urs immobilized PPCA surfaces at open circuit potential. The impedance spectra were fitted before and after immobilization of Urs using equivalent circuits of R(C(R(Q(RW)))). After immobilization of Urs onto the PPCA matrix, the charge transfer resistance (R-CT) increased for electrodes, which R-CT of urease immobilized PPCA-NaClO4 matrixes were lower than that of the Urs immobilized PPCA-Et4NBF4 matrixes indicating higher charge transfer ability. A decrease in double layer capacitance (C-dl) values when Urs was immobilized at the PPCA-NaClO4 electrode surface was observed, whereas an increase at the PPCA-Et4NBF4 electrode surface was observed. Urs immobilized electrodes resulted in a n-type semiconducting behavior revealing a positive slope. An increase in acceptor concentration of the electrodes, and a shift of the E-FB to more positive values with increasing amount of urease was observed. The Urs based electrode (PPCA-NaClO4-Urs) was used for detection of urea, which was detected in range of 0.9 to 14 mM with a detection limit (LOD) of 8.28 mu M. (C) 2016 The Electrochemical Society. All rights reserved.
dc.description.sponsorshipScientific & Technological Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [214Z200]
dc.description.sponsorshipThis work was supported by The Scientific & Technological Council of Turkey (TUBITAK)-214Z200 Project.
dc.identifier.doi10.1149/2.0671608jes
dc.identifier.endpageB444
dc.identifier.issn0013-4651
dc.identifier.issn1945-7111
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-84978148968
dc.identifier.scopusqualityQ1
dc.identifier.startpageB435
dc.identifier.urihttps://doi.org/10.1149/2.0671608jes
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7222
dc.identifier.volume163
dc.identifier.wosWOS:000379688000048
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKarazehir, Tolga
dc.institutionauthorAteş, Murat
dc.language.isoen
dc.publisherElectrochemical Soc Inc
dc.relation.ispartofJournal of the Electrochemical Society
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSolute-Solvent Interactions
dc.subjectElectrode Double-Layer
dc.subjectProtein Adsorption
dc.subjectSpectroscopy
dc.subjectComposite
dc.subjectPerformance
dc.subjectSupercapacitors
dc.subjectPolymerization
dc.subjectCapacitance
dc.subjectFilms
dc.titleCovalent Immobilization of Urease on Poly(Pyrrole-3-carboxylic Acid): Electrochemical Impedance and Mott Schottky Study
dc.typeArticle

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