Does Rainbow Trout Seminal Plasma-Cysteine Combination Affect the Cryo-Survivability and Post-Thaw Incubation Resilience of Ram Semen?

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dc.authoridGOKCE, ELIF/0000-0002-7678-3289
dc.contributor.authorGökçe, Elif
dc.contributor.authorÜstüner, Burcu
dc.date.accessioned2023-05-06T17:23:36Z
dc.date.available2023-05-06T17:23:36Z
dc.date.issued2022
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü, Dölerme ve Suni Tohumlama Ana Bilim Dalı
dc.description.abstractThe aim of this study was to evaluate the effect of both pure rainbow trout seminal plasma (RTSP) supplementation and RTSP-cysteine combination on cryopreservation success and post-thaw incubation resilience of ram semen in the nonbreeding season. For this purpose, different doses of RTSP (0%, 1%, 10%, and 15%) with or without cysteine supplementation were used for experiments. Ejaculates chosen for experiments were pooled and then divided into eight equal volumes for grouping (Control-ControlC, RTSP1-RTSP1C, RTSP10-RTSP10C, and RTSP15-RTSP15C). After cryopreservation, frozen-thawed semen samples were incubated for 5 hours at 37 degrees C for determination of post-thaw incubation resistance. Motility, HOST, TUNEL, Rh123-PI, and CTC tests were performed at 0 hour and 3rd and 5th hours of post-thaw incubation to evaluate the efficacy of all experimental groups. The RTSP10 and RTSP10C groups were noted to provide the best protection on motility, plasma membrane integrity, DNA integrity, and mitochondrial function of cryopreserved ram semen. On the other hand, the best protection against cryo-capacitation was observed in RTSP15 and RTSP15C groups. The addition of cysteine was found to be effective when the higher (15%) or lower (1%) doses of RTSP were used, as well as for no use of RTSP.
dc.description.sponsorshipUludag University Scientific Research Projects Unit, Bursa, Turkey (BAP) [DDP(V)-2017/10]
dc.description.sponsorshipThis work was supported by the Uludag University Scientific Research Projects Unit, Bursa, Turkey (BAP) (Project number: DDP(V)-2017/10)
dc.identifier.doi10.1089/bio.2022.0081
dc.identifier.issn1947-5535
dc.identifier.issn1947-5543
dc.identifier.pmid36516083
dc.identifier.scopus2-s2.0-85181176748
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1089/bio.2022.0081
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12191
dc.identifier.wosWOS:000898300400001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGökçe, Elif
dc.language.isoen
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofBiopreservation And Biobanking
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectcysteine
dc.subjectcryopreservation
dc.subjectincubation resilience
dc.subjectrainbow trout seminal plasma
dc.subjectram semen
dc.subjectLecithin-Based Extender
dc.subjectOncorhynchus-Mykiss
dc.subjectDna-Damage
dc.subjectSpermatozoa
dc.subjectSperm
dc.subjectQuality
dc.subjectCryopreservation
dc.subjectFrozen
dc.subjectProtein
dc.subjectCapacitation
dc.titleDoes Rainbow Trout Seminal Plasma-Cysteine Combination Affect the Cryo-Survivability and Post-Thaw Incubation Resilience of Ram Semen?
dc.typeArticle

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