TREATMENT WITH MELATONIN ENHANCES THE EMBRYO QUALITY AND THE DEVELOPMENT OF VITRIFIED/WARMED EIGHT CELL MOUSE EMBRYOS BY SOLID SURFACE VITRIFICATION (SSV)

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dc.authorid0000-0002-2789-0635
dc.authorwosidarat, sezen/ABA-7370-2020
dc.contributor.authorÇağlar, Kübra
dc.contributor.authorKocabay, Ahmet
dc.contributor.authorTaşkın, Ali Cihan
dc.contributor.authorArat, Sezen
dc.date.accessioned2022-05-11T14:02:45Z
dc.date.available2022-05-11T14:02:45Z
dc.date.issued2020
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.departmentEnstitüler, Fen Bilimleri Enstitüsü, Tarımsal Biyoteknoloji Ana Bilim Dalı
dc.description.abstractBACKGROUND: Melatonin is an endocrine hormone secreted from the pineal gland located outside the blood-brain barrier. OBJECTIVE: In this study, in vitro propagated eight-cell mouse embryos were vitrified by the Solid Surface Vitrification (SSV) method and after thawing, their in vitro development and embryo qualities in melatonin added media were investigated. METHODS: Pronuclear stage embryos obtained from super ovulated B6CBAF1/J strain mice, were cultured until the eight-cell stage. Then these eight-cell embryos were vitrified by the SSV method and after thawing, cultured in melatonin added media at 37 degrees C and 5 %CO2 conditions until the blastocyst stage. RESULT: In the experimental period, in vitro embryo development rates of the control, SSV and +10(-12) M melatonin groups were observed as 97%, 86% and 93%, respectively. CONCLUSION: Our results indicated that melatonin addition slightly increased the development rates and total cell numbers of embryos vitrified by the SSV method.
dc.description.sponsorshipRepublic of Turkey Ministry of DevelopmentTurkiye Cumhuriyeti Kalkinma Bakanligi; TUBITAK - The Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 114O638]
dc.description.sponsorshipThe authors gratefully acknowledge use of the services and facilities of the Koc University Research Center for Translational Medicine (KUTTAM), funded by the Republic of Turkey Ministry of Development. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Ministry of Development. This research was supported by a grant from TUBITAK - The Scientific and Technological Research Council of Turkey (Grant Number: TOVAG 114O638). This manuscript is derived from the Master's thesis of Kubra Caglar.
dc.identifier.endpage67
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue2en_US
dc.identifier.pmid33988655
dc.identifier.startpage62
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4468
dc.identifier.volume41
dc.identifier.wosWOS:000534629500002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorÇağlar, Kübra
dc.institutionauthorArat, Sezen
dc.language.isoen
dc.publisherCryo Letters
dc.relation.ispartofCryoletters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectmelatonin
dc.subjectvitrification
dc.subjectSSV
dc.subjectmouse
dc.subjecteight-cell stage
dc.subjectembryo development
dc.subjectGene-Expression Profiles
dc.subjectIn-Vitro
dc.subjectEquilibration Time
dc.subjectCulture-Medium
dc.subjectPronuclear
dc.subjectStage
dc.subjectSurvival
dc.subjectOocyte
dc.subjectCryopreservation
dc.subjectSupplementation
dc.titleTREATMENT WITH MELATONIN ENHANCES THE EMBRYO QUALITY AND THE DEVELOPMENT OF VITRIFIED/WARMED EIGHT CELL MOUSE EMBRYOS BY SOLID SURFACE VITRIFICATION (SSV)
dc.typeArticle

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