MAPK immunoreactivity in streptozotocin-induced diabetic rat testis

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dc.authorscopusid55134339900
dc.authorscopusid22134674200
dc.authorscopusid25643338300
dc.contributor.authorDönmez, Yeliz Bozdemir
dc.contributor.authorKizilay, G.
dc.contributor.authorTopcu-Tarladacalisir, Y.
dc.date.accessioned2022-05-11T14:41:27Z
dc.date.available2022-05-11T14:41:27Z
dc.date.issued2014
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.description.abstractPURPOSE: To evaluate the alterations of two mitogen-activated protein kinases (MAPK)s, extracellular signal regulated kinase (ERK) and c-Jun NH2 terminal kinase (JNK), in the testes of male rats with experimental diabetes.METHODS: Twenty males Sprague-Dawley rats were randomly divided into a control group (n=8) and a diabetes group (administration of 40 mg/kg/day streptozotocin (STZ) for five sequential days, n=12). After six weeks, testicular biopsy samples were obtained for light microscopy and immunohistochemical methods.RESULTS: The PCNA (proliferating cell nuclear antigen) index was significantly decreased in the diabetes group (p=0.004) when compared to the control group. Both total (t)-ERK and phosphor (p)-ERK immunoreactivities were significantly decreased in the diabetes group (p=0.004, p<0.001, respectively). The t-JNK immunoreactivity was unchanged in both groups (p=0.125), while p-JNK immunoreactivity was significantly increased in the diabetic group (p=0.002).CONCLUSIONS: The decrease of androgen levels in the course of diabetes may contribute to the decrease of the immunoreactivities of t-ERK and p-ERK. JNK may be activated due to the changes in various cytokines and chemochines that participate in the oxidative stress process of diabetes. Therefore, testicular apoptosis may occur and lead to infertility associated with diabetes. © 2014 Society for Neuroscience. All rights reserved.
dc.identifier.doi10.1590/S0102-8650201400160004
dc.identifier.endpage650
dc.identifier.issn0102-8650
dc.identifier.issue10en_US
dc.identifier.pmid25317996
dc.identifier.scopus2-s2.0-84908075354
dc.identifier.scopusqualityQ3
dc.identifier.startpage644
dc.identifier.urihttps://doi.org/10.1590/S0102-8650201400160004
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9185
dc.identifier.volume29
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDönmez, Yeliz Bozdemir
dc.language.isoen
dc.publisherSociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDiabetes mellitus
dc.subjectExtracellular signal-regulated kinases
dc.subjectJNK mitogen-activated protein kinases
dc.subjectMitogen-activated protein kinases
dc.subjectRats
dc.subjectStreptozocin
dc.subjectTestes
dc.subjectandrogen
dc.subjectcycline
dc.subjectglucose
dc.subjectinterleukin 1beta
dc.subjectinterleukin 6
dc.subjectmitogen activated protein kinase
dc.subjectstreptozocin
dc.subjectstress activated protein kinase
dc.subjecttumor necrosis factor alpha
dc.subjectmitogen activated protein kinase
dc.subjectstress activated protein kinase
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectdiabetes mellitus
dc.subjecthistology
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectmale
dc.subjectmale genital tract parameters
dc.subjectmicroscopy
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectquantitative analysis
dc.subjectrat
dc.subjectsignal transduction
dc.subjecttestis
dc.subjecttestis biopsy
dc.subjectWestern blotting
dc.subjectanimal
dc.subjectbiopsy
dc.subjectcomplication
dc.subjectevaluation study
dc.subjectexperimental diabetes mellitus
dc.subjectInfertility, Male
dc.subjectmetabolism
dc.subjectpathology
dc.subjectrandomization
dc.subjectWistar rat
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectBiopsy
dc.subjectDiabetes Mellitus, Experimental
dc.subjectExtracellular Signal-Regulated MAP Kinases
dc.subjectImmunohistochemistry
dc.subjectInfertility, Male
dc.subjectJNK Mitogen-Activated Protein Kinases
dc.subjectMale
dc.subjectRandom Allocation
dc.subjectRats, Wistar
dc.subjectStreptozocin
dc.subjectTestis
dc.titleMAPK immunoreactivity in streptozotocin-induced diabetic rat testis
dc.typeArticle

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