MAPK immunoreactivity in streptozotocin-induced diabetic rat testis
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Dosyalar
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
PURPOSE: To evaluate the alterations of two mitogen-activated protein kinases (MAPK)s, extracellular signal regulated kinase (ERK) and c-Jun NH2 terminal kinase (JNK), in the testes of male rats with experimental diabetes.METHODS: Twenty males Sprague-Dawley rats were randomly divided into a control group (n=8) and a diabetes group (administration of 40 mg/kg/day streptozotocin (STZ) for five sequential days, n=12). After six weeks, testicular biopsy samples were obtained for light microscopy and immunohistochemical methods.RESULTS: The PCNA (proliferating cell nuclear antigen) index was significantly decreased in the diabetes group (p=0.004) when compared to the control group. Both total (t)-ERK and phosphor (p)-ERK immunoreactivities were significantly decreased in the diabetes group (p=0.004, p<0.001, respectively). The t-JNK immunoreactivity was unchanged in both groups (p=0.125), while p-JNK immunoreactivity was significantly increased in the diabetic group (p=0.002).CONCLUSIONS: The decrease of androgen levels in the course of diabetes may contribute to the decrease of the immunoreactivities of t-ERK and p-ERK. JNK may be activated due to the changes in various cytokines and chemochines that participate in the oxidative stress process of diabetes. Therefore, testicular apoptosis may occur and lead to infertility associated with diabetes. © 2014 Society for Neuroscience. All rights reserved.
Açıklama
Anahtar Kelimeler
Diabetes mellitus, Extracellular signal-regulated kinases, JNK mitogen-activated protein kinases, Mitogen-activated protein kinases, Rats, Streptozocin, Testes, androgen, cycline, glucose, interleukin 1beta, interleukin 6, mitogen activated protein kinase, streptozocin, stress activated protein kinase, tumor necrosis factor alpha, mitogen activated protein kinase, stress activated protein kinase, animal experiment, animal model, animal tissue, apoptosis, Article, controlled study, diabetes mellitus, histology, immunohistochemistry, immunoreactivity, male, male genital tract parameters, microscopy, nonhuman, oxidative stress, quantitative analysis, rat, signal transduction, testis, testis biopsy, Western blotting, animal, biopsy, complication, evaluation study, experimental diabetes mellitus, Infertility, Male, metabolism, pathology, randomization, Wistar rat, Animals, Apoptosis, Biopsy, Diabetes Mellitus, Experimental, Extracellular Signal-Regulated MAP Kinases, Immunohistochemistry, Infertility, Male, JNK Mitogen-Activated Protein Kinases, Male, Random Allocation, Rats, Wistar, Streptozocin, Testis
Kaynak
Acta Cirurgica Brasileira
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
29
Sayı
10
