Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method

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dc.authorid0000-0003-1101-2296
dc.authorid0000-0002-2394-3795
dc.authorwosidKaplan, Selçuk/AAA-4359-2022
dc.authorwosidKaplan, Selçuk/ABA-2299-2020
dc.authorwosidGöçerler, Harun/AAS-2740-2020
dc.contributor.authorKaplan, Selçuk
dc.date.accessioned2022-05-11T14:42:33Z
dc.date.available2022-05-11T14:42:33Z
dc.date.issued2018
dc.departmentFakülteler, Veteriner Fakültesi, Zootekni ve Hayvan Besleme Bölümü, Genetik Ana Bilim Dalı
dc.description.abstractLeptin is a hormone which produces 16 kDa protein predominantly expressed in adipose tissue. Leptin gene has pleiotropic effects on several functional traits such as milk yield, carcass and meat quality, growth, feed intake and fertility. Therefore, this study was performed to identify single nucleotide polymorphism of bubaline leptin gene in Anatolian buffaloes. In this study, 513 bp of leptin DNA fragment was amplified. A PCR-RFLP analysis was used to identify bubaline leptin gene T1131G polymorphism. DdeI restriction enzyme was used to genotype bubaline leptin gene T1131G polymorphism in Anatolian buffaloes. The analysis revealed TT, GT and GG genotypes in Anatolian buffaloes. Allele frequencies of T and G were found 0.478 and 0.521, respectively. This study is the first report of identification of bubaline leptin gene T1131G polymorphism by using PCR-RFLP method in Anatolian buffaloes.
dc.identifier.doi10.28955/alinterizbd.402760
dc.identifier.endpage97
dc.identifier.issn2564-7814
dc.identifier.issn2587-2249
dc.identifier.issue1en_US
dc.identifier.startpage93
dc.identifier.urihttps://doi.org/10.28955/alinterizbd.402760
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9415
dc.identifier.volume33
dc.identifier.wosWOS:000457209600013
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.institutionauthorKaplan, Selçuk
dc.language.isoen
dc.publisherKastamonu Univ, Orman Fak
dc.relation.ispartofAlinteri Journal of Agriculture Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLeptin
dc.subjectAnatolian Buffalo
dc.subjectSNP
dc.subjectPCR-RFLP
dc.subjectSingle Nucleotide Polymorphism
dc.subjectMilk-Production Traits
dc.subjectMeat Quality Traits
dc.subjectWater-Buffalo
dc.subjectGrowth Traits
dc.subjectAssociation
dc.subjectCloning
dc.subjectMutation
dc.subjectCattle
dc.subjectDna
dc.titleCharacterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method
dc.typeArticle

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