Microarray analysis of high light intensity stress on hydrogen production metabolism of Rhodobacter capsulatus

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dc.authorscopusid35363944400
dc.authorscopusid6505961443
dc.authorscopusid7004598043
dc.authorscopusid7006258822
dc.contributor.authorGürgan, Muazzez
dc.contributor.authorKoku, H.
dc.contributor.authorEroğlu, I.
dc.contributor.authorYücel, M.
dc.date.accessioned2022-05-11T14:28:41Z
dc.date.available2022-05-11T14:28:41Z
dc.date.issued2020
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractBiohydrogen obtained from purple non sulfur bacteria (PNSB) is an environmentally friendly alternative for hydrogen production. PNSB can be employed in large scale outdoor photobioreactors to produce hydrogen by photofermentation with sunlight as the light source. In external environmental conditions, however, bacteria can experience stress due to high light intensities, which can inhibit or slow down hydrogen production. Previous studies with other PNSB showed varying responses to light intensities (above 4000 lux), in some cases improving, and in others adversely affecting hydrogen production. In this study, Rhodobacter capsulatus, a PNSB species that produce hydrogen efficiently from dark fermenter effluents containing acetate, was used to investigate the effects of high light intensity stress on the hydrogen production metabolism at the gene expression level. A microarray analysis was carried out using a custom-design Affymetrix GeneChip TR_RCH2a520699F. R. capsulatus DSM1710 was grown under a cyclic illumination of 2000 and 7000 lux (12 h light/12 h dark) in a hydrogen production medium having 30 mM acetate and 2 mM glutamate, and was exposed to a high light intensity (10,000 lux) for 1 h in the middle of a light period. The results reveal that photosynthetic reaction center genes were down-regulated in order to protect the photosynthetic membrane from damage. On the other hand, the expression of nitrogenase and electron transport system genes were enhanced by high light intensity. These results show that a high light intensity stress drives R. capsulatus to direct gene expression towards hydrogen production, which supports the hypothesis that hydrogen production is a way for the disposal of excess reducing equivalents to maintain the internal redox balance. © 2018
dc.description.sponsorship019825; Sixth Framework Programme, FP6; Orta Doğu Teknik Üniversitesi: 07-02-2013-005; Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK: 108T455
dc.description.sponsorshipThis study was a part of the PhD Thesis of Dr. Muazzez Gürgan. The authors kindly acknowledge the support from The Scientific and Technological Research Council of Turkey [Project No. 108T455 ], Middle East Technical University BAP Project [Project No. 07-02-2013-005 ], and European Commission-Research: The Sixth Framework Program for Research and Technological Development Sustainable Energy Systems EU FP6-SES IP HYVOLUTION [contract No. 019825 ]. The authors also thank to Middle East Technical University Central Lab Biotechnology and Molecular Biology R&D Center for the microarray facilities. This study was presented in 7th Global Conference on Global Warming 2018, Izmir, Turkey.
dc.description.sponsorshipThis study was a part of the PhD Thesis of Dr. Muazzez Gürgan. The authors kindly acknowledge the support from The Scientific and Technological Research Council of Turkey [Project No. 108T455], Middle East Technical University BAP Project [Project No. 07-02-2013-005], and European Commission-Research: The Sixth Framework Program for Research and Technological Development Sustainable Energy Systems EU FP6-SES IP HYVOLUTION [contract No. 019825]. The authors also thank to Middle East Technical University Central Lab Biotechnology and Molecular Biology R&D Center for the microarray facilities. This study was presented in 7th Global Conference on Global Warming 2018, Izmir, Turkey.
dc.identifier.doi10.1016/j.ijhydene.2018.12.205
dc.identifier.endpage3523
dc.identifier.issn0360-3199
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85060193001
dc.identifier.scopusqualityQ1
dc.identifier.startpage3516
dc.identifier.urihttps://doi.org/10.1016/j.ijhydene.2018.12.205
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6912
dc.identifier.volume45
dc.indekslendigikaynakScopus
dc.institutionauthorGürgan, Muazzez
dc.language.isoen
dc.publisherElsevier Ltd
dc.relation.ispartofInternational Journal of Hydrogen Energy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiohydrogen
dc.subjectGene expression
dc.subjectHigh light intensity
dc.subjectMicroarray
dc.subjectRhodobacter capsulatus
dc.subjectBacteria
dc.subjectElectron transport properties
dc.subjectGene expression
dc.subjectLight sources
dc.subjectMetabolism
dc.subjectMicroarrays
dc.subjectPhotosynthesis
dc.subjectBio-hydrogen
dc.subjectElectron transport systems
dc.subjectEnvironmental conditions
dc.subjectEnvironmentally friendly alternatives
dc.subjectHigh lights
dc.subjectPhotosynthetic reaction center
dc.subjectPurple non-sulfur bacteria
dc.subjectRhodobacter capsulatus
dc.subjectHydrogen production
dc.titleMicroarray analysis of high light intensity stress on hydrogen production metabolism of Rhodobacter capsulatus
dc.typeArticle

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