A disposable immunosensor using ITO based electrode modified by a star-shaped polymer for analysis of tumor suppressor protein p53 in human serum

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dc.authorid0000-0002-8716-9970
dc.authorscopusid55571487900
dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.authorwosidAYDIN, MUHAMMET/ABA-9027-2020
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidAYDIN, Elif Burcu/ABA-9053-2020
dc.contributor.authorAydın, Muhammet
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:12Z
dc.date.available2022-05-11T14:48:12Z
dc.date.issued2018
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractLabel-free immunosensor based on tetra armed star-shaped poly(glycidylmethacrylate) (Star(PGMA)) modified disposable ITO electrode was developed for detection of p53 protein, an important colorectal cancer biomarker. This disposable biosensor was fabricated by spin-coating technique using star-shaped Star(PGMA) with epoxy side groups. After formation of a stable film with epoxy ends, anti-p53 antibodies were bound to these groups covalently. Stepwise modification of the electrode was followed by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) studies. The electrochemical performance of the immunosensor was studied by EIS. Furthermore, the antibody and antigen coupling was monitored by single frequency impedance (SFI) technique. The immunosensor showed a low limit of detection (7 fg/mL) and a linear detection range between 0.02 pg/mL and 4 pg/mL. Additionally, atomic force microscopy (AFM) and scanning electron microscopy (SEM) were utilized for monitoring of immunosensor surface at different stages of fabrication. The antibody coupling on the electrode surface was proved by Fourier-transform infrared spectroscopy (FTIR) and Raman spectroscopy. Furthermore, the proposed immunosensor had good reproducibility and repeatability.
dc.description.sponsorshipScientific and Technological Research Council of by Namik Kemal University, NKU-BAP [NKUBAP.00.DS.17.114]
dc.description.sponsorshipThis research was supported by The Scientific and Technological Research Council of by Namik Kemal University, NKU-BAP (Grant number: NKUBAP.00.DS.17.114).
dc.identifier.doi10.1016/j.bios.2018.02.017
dc.identifier.endpage9
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.pmid29425857
dc.identifier.scopus2-s2.0-85041465626
dc.identifier.scopusqualityQ1
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1016/j.bios.2018.02.017
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10612
dc.identifier.volume107
dc.identifier.wosWOS:000428006900001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Muhammet
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier Advanced Technology
dc.relation.ispartofBiosensors & Bioelectronics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectProtein p53
dc.subjectElectrochemical impedance spectroscopy
dc.subjectCyclic voltammetry
dc.subjectSingle frequency impedance
dc.subjectStar-polymer
dc.subjectPoly(glycidyl methacrylate)
dc.subjectConducting Polymer
dc.subjectRaman-Spectroscopy
dc.subjectLabel-Free
dc.subjectCancer
dc.subjectBiosensor
dc.subjectBiomarkers
dc.subjectDiagnosis
dc.subjectBlood
dc.titleA disposable immunosensor using ITO based electrode modified by a star-shaped polymer for analysis of tumor suppressor protein p53 in human serum
dc.typeArticle

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