Nuclear DNA content and ploidy levels of living apple germplasm collection in Türkiye

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dc.contributor.authorKacal, Emel
dc.contributor.authorSimsek, Yaren Ipek
dc.contributor.authorSeymen, Turgay
dc.contributor.authorOezongun, Serif
dc.contributor.authorTuna, Metin
dc.date.accessioned2024-10-29T17:59:47Z
dc.date.available2024-10-29T17:59:47Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractOne of the key uses of flow cytometry is the estimation of nuclear DNA content and ploidy level, which has proven a reliable and effective approach in many studies. The primary goal of this research is to estimate the nuclear DNA content and ploidy level of the apple genetic resource collection maintained in the Fruit Research Institute's living Malus collection for the first time using flow cytometry (for autochthonous varieties). Fresh apple leaf tissues were used for the flow cytometry analysis. For each genotype, nuclear DNA analysis was performed on three individual plants. Propidium iodide (PI) is used as a fluorochrome. Common vetch (3.65 pg/2C) was used as an internal standard. The 2C nuclear DNA content ranged from 1.46 pg to 2.45 pg. The variation in nuclear DNA content within the collection was statistically significant. Apple genotypes were split into two groups, diploid and triploid, according to their nuclear DNA content. Based on these results, 16.47% of apple genotypes were triploid, while 83.53% were diploid. The 2C nuclear DNA content in triploid genotypes varied from 2.04 to 2.45 pg and in diploids from 1.46 to 1.69 pg. The average nuclear DNA content in diploids was 1.56 pg, whereas it was 2.29 pg in triploids. The results of the study will be useful to determine the best strategies in breeding programs, as ploidy is one of the most important characteristics to consider in selecting parents for breeding purposes in addition to their characteristics.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK) [219O095]
dc.description.sponsorshipThe authors would like to thank The Scientific and Technological Research Council of Turkiye (TUBITAK) [project 219O095] for their financial support.
dc.identifier.doi10.55730/1300-008X.2771
dc.identifier.endpage341
dc.identifier.issn1300-008X
dc.identifier.issn1303-6106
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85173843933
dc.identifier.scopusqualityQ2
dc.identifier.startpage331
dc.identifier.trdizinid#BAŞV!
dc.identifier.urihttps://doi.org/10.55730/1300-008X.2771
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1208234
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14838
dc.identifier.volume47
dc.identifier.wosWOS:001080468600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMalus x domestica
dc.subjectgenetic resources
dc.subjectchromosome number
dc.subjectflow cytometry
dc.subjectnuclear DNA content
dc.subjectploidy
dc.titleNuclear DNA content and ploidy levels of living apple germplasm collection in Türkiye
dc.title.alternativeNuclear DNA content and ploidy levels of living apple germplasm collection in Türkiye
dc.typeArticle

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