Single Nucleotide Polymorphism of Ovine Leptin and Insulin-Like Growth Factor 1 Gene in Kivircik Crossbred Ewes

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dc.authorid0000-0003-4942-7729
dc.authorid0000-0002-2394-3795
dc.authorid0000-0003-1101-2296
dc.authorwosidatalay, sertaç/ABA-9134-2020
dc.authorwosidKaplan, Selçuk/ABA-2299-2020
dc.authorwosidKaplan, Selçuk/AAA-4359-2022
dc.authorwosidGöçerler, Harun/AAS-2740-2020
dc.contributor.authorKaplan, Selçuk
dc.contributor.authorAtalay, Sertaç
dc.date.accessioned2022-05-11T14:15:40Z
dc.date.available2022-05-11T14:15:40Z
dc.date.issued2018
dc.departmentFakülteler, Veteriner Fakültesi, Zootekni ve Hayvan Besleme Bölümü, Genetik Ana Bilim Dalı
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractKivircik crossbred sheep in the Thrace region are commonly grown for meat production. The objective of the present study was to determine the polymorphism of insulin-like growth factor 1 (IGF1) and leptin (LEP) genes in Kivircik crossbred ewes. Therefore, IGF1/BfoI and Lep/BcnI polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism method. The single nucleotide polymorphism (SNP) in the regulatory region of the IGF1 gene was detected by amplification of the 294 bp region using specific primers and cleavage with the BfoI enzyme. Allele frequencies of A and B were found with 0.915 and 0.085 respectively. The genotype frequencies of IGF1 gene were 0.85 (AA), 0.13 (AB) and 0.02 (BB). The SNP in the exon 3 of the LEP gene was detected by amplification of the 494 bp region using specific primers and cleavage with the BcnI enzyme. The estimated frequencies of three genotypes including GG, GA and AA at Lep/BcnI polymorphism were 0.90, 0.09 and 0.01 and they were 0.055 and 0.945 for A and G alleles, respectively. LEP and IGF1 gene showed polymorphic patterns in Kivircik crossbred sheep population. There was no deviation from Hardy-Weinberg equilibrium (P>0.05) relative to LEP and IGF1 genotypes.
dc.description.sponsorshipCommission of Scientific Research Projects of Namik Kemal University in Turkey [NKUBAP.10.GA.17.117]
dc.description.sponsorshipThis study has been supported by the project numbered as NKUBAP.10.GA.17.117 accepted by Commission of Scientific Research Projects of Namik Kemal University in Turkey. We are thankfull to Lider Meat Ipsala company for providing tissue samples.
dc.identifier.doi10.17582/journal.pjz/2018.50.3.851.856
dc.identifier.endpage856
dc.identifier.issn0030-9923
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85050726034
dc.identifier.scopusqualityQ4
dc.identifier.startpage851
dc.identifier.urihttps://doi.org/10.17582/journal.pjz/2018.50.3.851.856
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6021
dc.identifier.volume50
dc.identifier.wosWOS:000447589600008
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKaplan, Selçuk
dc.institutionauthorAtalay, Sertaç
dc.language.isoen
dc.publisherZoological Soc Pakistan
dc.relation.ispartofPakistan Journal of Zoology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectLeptin
dc.subjectInsulin Like Growth Factor 1
dc.subjectSingle nucleotide polymorphism
dc.subjectSNP
dc.subjectIgf-I Gene
dc.subjectPolymerase-Chain-Reaction
dc.subjectCarcass Traits
dc.subjectAssociation
dc.subjectSheep
dc.subjectQuality
dc.subjectDimensions
dc.subjectSize
dc.titleSingle Nucleotide Polymorphism of Ovine Leptin and Insulin-Like Growth Factor 1 Gene in Kivircik Crossbred Ewes
dc.typeArticle

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