Investigation of the Potential Effects of Alpha-Lipoic Acid on Human Degenerated Intervertebral Disc Tissue Primary Cell Cultures

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dc.authoridÖzbek, Hanefi/0000-0002-8084-7855
dc.authoridAYDIN, IRFAN/0000-0001-5488-2262
dc.authorscopusid57703061100
dc.authorscopusid56769801000
dc.authorscopusid56674583800
dc.authorscopusid7005912992
dc.authorwosidÖzbek, Hanefi/O-3472-2019
dc.authorwosidAYDIN, IRFAN/AAK-9913-2021
dc.contributor.authorAydın, İrfan
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorÖzbek, Hanefi
dc.date.accessioned2023-04-20T08:01:12Z
dc.date.available2023-04-20T08:01:12Z
dc.date.issued2022
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractAIM: To investigate the supplementation of alpha-lipoic acid (ALA) at the molecular level to determine its effect on primary cell cultures prepared from human intervertebral disc (IVD) tissue in an in vitro environment. MATERIAL and METHODS: Human primary cell cultures were prepared from IVD tissue resected during surgery. While cell cultures without ALA supplementation formed the control group, those with ALA supplementation formed the study group. All cell groups were stained using acridine orange/propidium iodide (AO/PI), and the incidence of apoptotic cell death was determined under a fluorescent microscope. Cell surface morphology and extracellular matrix (ECM) structures were evaluated under an invert light microscope. Simultaneously, cell proliferation was evaluated by MTT???ELISA analysis, and the expressions of chondroadherin (CHAD), cartilage oligomeric protein (COMP), interleukin-1 beta (IL-1??), and matrix metalloproteinase (MMP)-7 and-19, which are genes associated with ECM regulation, were tested using qRT???PCR. The data obtained were evaluated statistically using Tukey???s honestly significant difference (HSD) test after analysis of variance (ANOVA) was performed. The alpha significance value was accepted as < .05. RESULTS: Compared to the cells in the control group, it was observed that both proliferation was suppressed and ECM structures deteriorated in the cells in the study group. CONCLUSION: Also, it was reported that the all-gene expression levels changed. ALA supplementation can negatively affect human IVD primary cell cultures in an in vitro environment.
dc.identifier.doi10.5137/1019-5149.JTN.34917-21.3
dc.identifier.endpage429
dc.identifier.issn1019-5149
dc.identifier.issue3en_US
dc.identifier.pmid35147963
dc.identifier.scopus2-s2.0-85130493382
dc.identifier.scopusqualityQ3
dc.identifier.startpage420
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.34917-21.3
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10795
dc.identifier.volume32
dc.identifier.wosWOS:000804648400012
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorYaşar Şirin, Duygu
dc.language.isoen
dc.publisherTurkish Neurosurgical Soc
dc.relation.ispartofTurkish Neurosurgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAlpha-Lipoic Acid
dc.subjectInterleukin-1 Beta
dc.subjectIntervertebral Disc
dc.subjectCartilage Oligomeric Matrix Protein
dc.subjectChondroadherin Gene
dc.subjectMatrix Metalloproteinase
dc.subjectAcetyl-L-Carnitine
dc.subjectHippocampal Tissue
dc.subjectNucleus Pulposus
dc.subjectPain
dc.subjectProliferation
dc.subjectProtein
dc.subjectGene
dc.titleInvestigation of the Potential Effects of Alpha-Lipoic Acid on Human Degenerated Intervertebral Disc Tissue Primary Cell Cultures
dc.typeArticle

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