A novel impedimetric disposable immunosensor for rapid detection of a potential cancer biomarker

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dc.authorid0000-0002-6232-3388
dc.authorscopusid25935968900
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidAsav, Engin/AAG-1170-2021
dc.authorwosidAsav, Engin/AAG-4182-2021
dc.contributor.authorAsav, Engin
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:29:52Z
dc.date.available2022-05-11T14:29:52Z
dc.date.issued2014
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractA specific and sensitive biosensor was developed successfully for quantitative detection of human epidermal growth factor receptor by electrochemical impedance spectroscopy. Anti-human epidermal growth factor receptor antibody was covalently immobilized onto a screen-printed carbon electrode modified with a carbon nanotube. Immobilization steps were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). After human epidermal growth factor receptor ligates with anti-human epidermal growth factor receptor immobilized onto an electrode surface, charge transfer resistance changes considerably. This electrochemical response was correlated with human epidermal growth factor receptor concentration. Under optimal conditions, the proposed biosensor could detect human epidermal growth factor receptor 2 fg/mL with a linear range from 2 to 14 fg/mL, showing high sensitivity. Kramers-Kronig Transform was performed on the experimental impedance data. Meanwhile, in a biosensor system, the Single Frequency Impedance technique was first used for characterization of interaction between human epidermal growth factor receptor and anti-human epidermal growth factor receptor. Eventually, the proposed biosensor was applied to artificial serum samples spiked with human epidermal growth factor receptor. (C) 2014 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109T 172]
dc.description.sponsorshipSupport from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 109T 172) is greatly acknowledged. Also we are grateful to Namik Kemal University Scientific and Technological Research Center (NABILTEM).
dc.identifier.doi10.1016/j.ijbiomac.2014.02.032
dc.identifier.endpage280
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid24560951
dc.identifier.scopus2-s2.0-84900354655
dc.identifier.scopusqualityQ1
dc.identifier.startpage273
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2014.02.032
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7159
dc.identifier.volume66
dc.identifier.wosWOS:000335201900036
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCancer biomarkers
dc.subjectBiosensor
dc.subjectHuman epidermal growth factor receptor
dc.subjectElectrochemical impedance spectroscopy
dc.subjectSingle frequency impedance
dc.subjectGrowth-Factor Receptor
dc.subjectActivated Carbon
dc.subjectSensitive Detection
dc.subjectSurface-Chemistry
dc.subjectProtein
dc.subjectAdsorption
dc.subjectExpression
dc.subjectHer-3
dc.subjectNanoparticles
dc.subjectDispersion
dc.titleA novel impedimetric disposable immunosensor for rapid detection of a potential cancer biomarker
dc.typeArticle

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