Glucose oxidase immobilization onto Au/poly[anthranilic acid-co-3-carboxy-N-(2-thenylidene)aniline]/PVAc electrospun nanofibers

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dc.authorid0000-0002-1806-0330
dc.authorid0000-0001-7513-1740
dc.authorscopusid56543790200
dc.authorscopusid54941444900
dc.authorscopusid34167906800
dc.authorscopusid9735216100
dc.authorscopusid35548936600
dc.authorwosidgolshaei, rana/AAP-1260-2021
dc.authorwosidAtes, Murat/G-3798-2012
dc.authorwosidSarac, A.Sezai/N-1628-2014
dc.contributor.authorGolshaei, Rana
dc.contributor.authorKarazehir, Tolga
dc.contributor.authorGhoreishi, Sayed Mehdi
dc.contributor.authorAteş, Murat
dc.contributor.authorSaraç, A. Sezai
dc.date.accessioned2022-05-11T14:30:56Z
dc.date.available2022-05-11T14:30:56Z
dc.date.issued2017
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractAu/poly[anthranilic acid-co-3-carboxy-N-(2-thenylidene)aniline/PVAc] [Au/P(ANA-co-CNTA)/PVAc] electrospun nanofibers were fabricated in different electrospinning media including acetone and dimethylformamide (DMF) for covalent immobilization of glucose oxidase (GOx). The surface of copolymer nanofibers was activated by EDC/NHS chemistry, and the presence of Au nanoparticles as tiny conduction centers inside the copolymer matrix enhanced the electrochemical properties. Morphology and composition of enzyme-immobilized nanofibers were characterized by scanning electron microscopy/energy-dispersive X-ray spectroscopy (EM/EDX) and atomic force microscope (AFM). The effective covalent binding of glucose oxidase onto the Au/P(ANA-co-CNTA)/PVAc nanofibers was also confirmed by FTIR-ATR and Raman spectroscopy. EIS measurements revealed that the charge transfer resistances of the enzyme-immobilized nanofibers were decreased with increasing amount of enzyme. The effect of electrospun nanofiber diameter on sensing properties of enzyme-functionalized nanofibers was investigated by EIS. The sensitivities of electrodes calculated from impedance measurement were 7.24 x 10(6) and 6.67 x 10(3) Omega mM(-1) cm(-2) for the Au/P(ANA-co-CNTA)/PVAc-GO(X) (DMF) and Au/P(ANA-co-CNTA)/PVAc-GO(X) (acetone), respectively. The impedance measurement results revealed that the linear range of Au/P(ANA-co-CNTA)/PVAc-GO(X) (DMF) was lower than Au/P(ANA-co-CNTA)/PVAc-GO(X) (acetone). It could be attributed that smaller fiber diameter resulted in the higher specific surface area. This contributes to increasing the number of available active sites and, thus, increasing the amount of the enzyme loading.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2216]
dc.description.sponsorshipThe authors express their thanks to Scientific and Technological Research Council of Turkey (TUBITAK) for the financial support on Project 2216. We would like to thank Zeliha Guler for her valuable discussions.
dc.identifier.doi10.1007/s00289-016-1786-0
dc.identifier.endpage1517
dc.identifier.issn0170-0839
dc.identifier.issn1436-2449
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-84982242386
dc.identifier.scopusqualityQ2
dc.identifier.startpage1493
dc.identifier.urihttps://doi.org/10.1007/s00289-016-1786-0
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7244
dc.identifier.volume74
dc.identifier.wosWOS:000399176700005
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKarazehir, Tolga
dc.institutionauthorAteş, Murat
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPolymer Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectNanofibers
dc.subjectCovalent immobilization
dc.subjectGlucose oxidase
dc.subjectElectrochemical impedance spectroscopy
dc.subjectElectrochemical biosensing of glucose
dc.subjectElectrochemical Impedance
dc.subjectGold Nanoparticles
dc.subjectBiosensor Applications
dc.subjectPolythiophene
dc.subjectPolypyrrole
dc.subjectFabrication
dc.subjectGraphene
dc.subjectEnzymes
dc.subjectMatrix
dc.subjectSensor
dc.titleGlucose oxidase immobilization onto Au/poly[anthranilic acid-co-3-carboxy-N-(2-thenylidene)aniline]/PVAc electrospun nanofibers
dc.typeArticle

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