Analysis of Tau-441 protein in clinical samples using rGO/AuNP nanocomposite-supported disposable impedimetric neuro-biosensing platform: Towards Alzheimer's disease detection

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dc.authorscopusid56800873300
dc.authorscopusid6506764918
dc.contributor.authorSonuç Karaboğa, Münteha Nur
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:27Z
dc.date.available2022-05-11T14:07:27Z
dc.date.issued2020
dc.departmentYüksekokullar, Sağlık Yüksekokulu, Beslenme ve Diyetetik Bölümü
dc.description.abstractChanges in isoforms of Tau protein, which are critical for microtubule functioning, are accepted as being responsible for diseases characterized by dementia, in particular Alzheimer's disease (AD). In this comprehensive study, a single-use neuro-biosensing probe for the determination of Tau-441 protein was developed by utilizing the power of nanocomposites consisting of reduced graphene oxide (rGO) and gold nanoparticles (AuNP) using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The nanocomposite surface (rGO-AuNP) was modified with 11-mercaptoundecanoic acid (11-MUA) act as covalent anchorer to increase the sensitivity of the assay. Surface coverage value and pinhole ratio were calculated using EIS data. Kramers-kronig data, which helps to interpret instrumental errors, are also calculated. The immunoreaction of Tau-441 with anti-Tau was monitored simultaneously with Single Frequency Impedance (SFI). The changes in surface morphology were evaluated with scanning electron microscopy (SEM), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). The designed immunosensor showed a linear response within the concentration range of 1–500 pg/mL for the target analyte Tau-441 and the limit of detection was found to be 0.091 pg/mL. The promising point of the study is that this neuro-biosensor system can capture the Tau-441 target protein in both serum fluid and cerebrospinal fluid (CSF) samples with recoveries ranging from 96% to 108%. © 2020 Elsevier B.V.
dc.identifier.doi10.1016/j.talanta.2020.121257
dc.identifier.issn0039-9140
dc.identifier.pmid32887148
dc.identifier.scopus2-s2.0-85086586129
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2020.121257
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5111
dc.identifier.volume219
dc.identifier.wosWOS:000568747200003
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSonuç Karaboğa, Münteha Nur
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAlzheimer’ disease
dc.subjectBiosensor
dc.subjectElectrochemical impedance spectroscopy
dc.subjectPinhole
dc.subjectReduced graphene oxide
dc.subjectTau
dc.subjectCyclic voltammetry
dc.subjectElectrochemical impedance spectroscopy
dc.subjectFourier transform infrared spectroscopy
dc.subjectGold nanoparticles
dc.subjectGraphene
dc.subjectMorphology
dc.subjectNeurodegenerative diseases
dc.subjectProteins
dc.subjectReduced Graphene Oxide
dc.subjectScanning electron microscopy
dc.subjectSurface morphology
dc.subject11-mercaptoundecanoic acid
dc.subjectAlzheimer's disease
dc.subjectBio-sensor systems
dc.subjectBiosensing platforms
dc.subjectCerebro spinal fluids
dc.subjectConcentration ranges
dc.subjectInstrumental error
dc.subjectReduced graphene oxides (RGO)
dc.subjectCerebrospinal fluid
dc.subjectgold
dc.subjectgraphene oxide
dc.subjectgraphite
dc.subjectmetal nanoparticle
dc.subjectnanocomposite
dc.subjecttau protein
dc.subjectAlzheimer disease
dc.subjectelectrode
dc.subjectgenetic procedures
dc.subjecthuman
dc.subjectimmunoassay
dc.subjectAlzheimer Disease
dc.subjectBiosensing Techniques
dc.subjectElectrodes
dc.subjectGold
dc.subjectGraphite
dc.subjectHumans
dc.subjectImmunoassay
dc.subjectMetal Nanoparticles
dc.subjectNanocomposites
dc.subjecttau Proteins
dc.titleAnalysis of Tau-441 protein in clinical samples using rGO/AuNP nanocomposite-supported disposable impedimetric neuro-biosensing platform: Towards Alzheimer's disease detection
dc.typeArticle

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