Analysis of Tau-441 protein in clinical samples using rGO/AuNP nanocomposite-supported disposable impedimetric neuro-biosensing platform: Towards Alzheimer's disease detection
Yükleniyor...
Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Changes in isoforms of Tau protein, which are critical for microtubule functioning, are accepted as being responsible for diseases characterized by dementia, in particular Alzheimer's disease (AD). In this comprehensive study, a single-use neuro-biosensing probe for the determination of Tau-441 protein was developed by utilizing the power of nanocomposites consisting of reduced graphene oxide (rGO) and gold nanoparticles (AuNP) using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The nanocomposite surface (rGO-AuNP) was modified with 11-mercaptoundecanoic acid (11-MUA) act as covalent anchorer to increase the sensitivity of the assay. Surface coverage value and pinhole ratio were calculated using EIS data. Kramers-kronig data, which helps to interpret instrumental errors, are also calculated. The immunoreaction of Tau-441 with anti-Tau was monitored simultaneously with Single Frequency Impedance (SFI). The changes in surface morphology were evaluated with scanning electron microscopy (SEM), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). The designed immunosensor showed a linear response within the concentration range of 1–500 pg/mL for the target analyte Tau-441 and the limit of detection was found to be 0.091 pg/mL. The promising point of the study is that this neuro-biosensor system can capture the Tau-441 target protein in both serum fluid and cerebrospinal fluid (CSF) samples with recoveries ranging from 96% to 108%. © 2020 Elsevier B.V.
Açıklama
Anahtar Kelimeler
Alzheimer’ disease, Biosensor, Electrochemical impedance spectroscopy, Pinhole, Reduced graphene oxide, Tau, Cyclic voltammetry, Electrochemical impedance spectroscopy, Fourier transform infrared spectroscopy, Gold nanoparticles, Graphene, Morphology, Neurodegenerative diseases, Proteins, Reduced Graphene Oxide, Scanning electron microscopy, Surface morphology, 11-mercaptoundecanoic acid, Alzheimer's disease, Bio-sensor systems, Biosensing platforms, Cerebro spinal fluids, Concentration ranges, Instrumental error, Reduced graphene oxides (RGO), Cerebrospinal fluid, gold, graphene oxide, graphite, metal nanoparticle, nanocomposite, tau protein, Alzheimer disease, electrode, genetic procedures, human, immunoassay, Alzheimer Disease, Biosensing Techniques, Electrodes, Gold, Graphite, Humans, Immunoassay, Metal Nanoparticles, Nanocomposites, tau Proteins
Kaynak
Talanta
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
219
