Determination of DGAT1 K232A Polymorphism in Donkey Populations Reared in Thrace Region of Turkey by DNA Sequencing

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dc.authorscopusid57200696504
dc.authorscopusid26028615800
dc.contributor.authorIşık, Raziye
dc.contributor.authorÖzdil, Fulya
dc.date.accessioned2022-05-11T14:07:11Z
dc.date.available2022-05-11T14:07:11Z
dc.date.issued2019
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractDiacylglycerol acyltransferase 1 (DGAT1) gene has become a potential candidate gene for milk composition and yield in cattle. A nonconservative lysine to alanine substitution (K232A) at position 10.433 and 10.434 in the eighth exon of DGAT1 gene has widely studied in cattle, goat, and sheep. This substitution conducted a considerable effect on milk fat, which was previously mapped to the centromeric end of bovine chromosome 14. Yet, no information has been found about DGAT1 gene in donkeys. In this study, the genetic variation of DGAT1 gene has been analyzed by CfrI (EaeI) restriction and DNA sequencing in 85 donkeys reared in Thrace region of Turkey. No digestion is found in the DGAT1 gene via CfrI restriction enzyme in the studied donkey populations. The DNA sequence of 437 bp of the DGAT1 gene in donkeys revealed only Alanine allele (K232A), which was reported in cattle with high milk fat content. But we found a novel polymorphism at position 10.435, from G to A, which also revealed Alanine allele. As a conclusion, CfrI digestion is not a diagnostic site in DGAT1 gene in donkeys. The DNA sequence of DGAT1 gene in donkeys is reported for the first time in this study, and this sequence is deposited to National Center for Biotechnology Information GenBank database with the accession number MK086162. © 2018 Elsevier Inc.
dc.description.sponsorshipNKUBAP.03
dc.description.sponsorshipFinancial disclosure: The Scientific Research Projects Coordination Unit of Namık Kemal University, Turkey (Project No: NKUBAP.03.DS.17.129, Project Leader: Raziye IŞIK).
dc.identifier.doi10.1016/j.jevs.2018.11.012
dc.identifier.endpage50
dc.identifier.issn0737-0806
dc.identifier.scopus2-s2.0-85058173936
dc.identifier.scopusqualityQ2
dc.identifier.startpage48
dc.identifier.urihttps://doi.org/10.1016/j.jevs.2018.11.012
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4998
dc.identifier.volume73
dc.identifier.wosWOS:000456711400008
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorIşık, Raziye
dc.institutionauthorÖzdil, Fulya
dc.language.isoen
dc.publisherW.B. Saunders
dc.relation.ispartofJournal of Equine Veterinary Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDGAT1 gene
dc.subjectDNA sequencing
dc.subjectDonkey
dc.subjectEquus asinus
dc.subjectTurkey
dc.subjectdiacylglycerol acyltransferase 1
dc.subjectgenomic DNA
dc.subjectmilk fat
dc.subjectrestriction endonuclease
dc.subjectallele
dc.subjectanimal cell
dc.subjectArticle
dc.subjectbovine
dc.subjectDNA extraction
dc.subjectDNA sequence
dc.subjectdonkey
dc.subjectgene amplification
dc.subjectgene sequence
dc.subjectgenetic polymorphism
dc.subjectgenetic variation
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpolymerase chain reaction
dc.subjectrestriction fragment
dc.subjectrestriction fragment length polymorphism
dc.titleDetermination of DGAT1 K232A Polymorphism in Donkey Populations Reared in Thrace Region of Turkey by DNA Sequencing
dc.typeArticle

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