A label-free electrochemical biosensor for direct detection of RACK 1 by using disposable, low-cost and reproducible ITO based electrode

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dc.authorscopusid57201802689
dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidAYDIN, Elif Burcu/ABA-9053-2020
dc.contributor.authorTörer, Hakan
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:12:20Z
dc.date.available2022-05-11T14:12:20Z
dc.date.issued2018
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractIn this study we designed an ultrasensitive electrochemical immunosensor for RACK 1 detection using 11-cyanoundecyltrimethoxysilane (11-CUTMS) as a immobilization matrix to immobilize biorecognition element. The used silane agent (11-CUTMS) provides a favorable platform for efficient loading of anti-RACK 1 antibody. The effective loading of the biorecognition element on the 11-CUTMS matrix was monitored by scanning electron microscopy (SEM), atomic force microscopy (AFM) images and fourier transform infrared spectroscopy (FTIR) spectra. The electrochemical characterization of the immunosensor was performed by using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Moreover, biorecognition interaction between anti-RACK1 antibodies and RACK1 antigens was monitored by using single frequency technique (SFI). The operating conditions, calibration curves obtained during optimization of experiments and reproducibility of the proposed impedimetric RACK1 biosensor are also investigated and discussed. The electrochemical immunosensor illustrated a sensitive response to RACK 1 antigen with detection limit of 10.8 fg/mL and in the linear range of 0.036 -2.278 pg/mL (R-2 = 0.999). Owing to high specificity, good reproducibility, long stability and reusability, the fabricated immunosensor will provide a sensitive, selective approach to RACK 1 detection. Furthermore, the practical applicability in human serum samples were investigated with a satisfactory result. (C) 2018 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipThe financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged.
dc.identifier.doi10.1016/j.aca.2018.04.031
dc.identifier.endpage72
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.pmid29776548
dc.identifier.scopus2-s2.0-85046151070
dc.identifier.scopusqualityQ1
dc.identifier.startpage65
dc.identifier.urihttps://doi.org/10.1016/j.aca.2018.04.031
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5508
dc.identifier.volume1024
dc.identifier.wosWOS:000432363600005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTörer, Hakan
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectReceptor of activated C kinase
dc.subjectRACK1
dc.subject11-cyanoundecyltrimethoxysilane
dc.subjectITO
dc.subjectBiosensor
dc.subjectFree Impedimetric Biosensor
dc.subjectSelf-Assembled Monolayer
dc.subjectC-Kinase 1
dc.subjectImpedance Spectroscopy
dc.subjectBiocatalytic Precipitation
dc.subjectImmunosensor
dc.subjectCancer
dc.subjectBiomarker
dc.subjectSensor
dc.subjectFilm
dc.titleA label-free electrochemical biosensor for direct detection of RACK 1 by using disposable, low-cost and reproducible ITO based electrode
dc.typeArticle

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