MicroRNA-17-5p targets expression of cancer-associated genes in breast cancer cells

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dc.authorscopusid56497061100
dc.contributor.authorBozgeyik, Esra
dc.date.accessioned2022-05-11T14:05:12Z
dc.date.available2022-05-11T14:05:12Z
dc.date.issued2020
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.description.abstractAlthough there are several studies, biological function and therapeutic potential of miR-17-5p in breast cancer carcinogenesis remains muchly elusive. Specifically, its interaction with several cancer-associated genes remains unexplored in breast cancer. Here, we aimed to demonstrate the biological function and therapeutic potential of miR-17-5p in breast cancer through determination of the interactions between cancer-associated genes and miR-17-5p. MFC-7 breast cancer cells used in the study and cells were transfected with miR-17-5p miRNA mimics to ectopically overexpress miR-17-5p. MiR-17-5p expression levels and expression changes of cancer-associated genes were determined by using qPCR method. Expression levels of miR-17-5p were significantly enhanced following 72 h of mimic transfections as compared to scrambled control and blank control. Overexpression of miR-17-5p led to differential expression of several cancer-associated genes. Notably, BECN, CDKN2B and AIFM genes were significantly altered. Although not significant, expression levels of BAX, BCLXL, VIM, BCL2, MTOR, RB1, AKT1, XIAP, P53, and PTEN genes were found to be slightly elevated whereas expression levels of BCL-2, VIM and AKT1 were found to be decreased. Results of the present study indicate that miR-17-5p can act as both tumor suppressor and tumor promoter oncomiR in breast cancer. © 2019 Elsevier B.V.
dc.identifier.doi10.1016/j.mgene.2019.100614
dc.identifier.issn2214-5400
dc.identifier.scopus2-s2.0-85073827804
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://doi.org/10.1016/j.mgene.2019.100614
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4919
dc.identifier.volume24
dc.identifier.wosWOS:000525750700043
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorBozgeyik, Esra
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofMeta Gene
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAIFM
dc.subjectBECN
dc.subjectBreast cancer
dc.subjectCDKN2B
dc.subjectmiR-17-5p
dc.subjectmiRNA
dc.subjectapoptosis inducing factor
dc.subjectbeclin 1
dc.subjectcyclin dependent kinase inhibitor 2B
dc.subjectmammalian target of rapamycin
dc.subjectmicroRNA
dc.subjectmicrorna 17 5p
dc.subjectphosphatidylinositol 3,4,5 trisphosphate 3 phosphatase
dc.subjectprotein Bax
dc.subjectprotein bcl 2
dc.subjectprotein bcl xl
dc.subjectprotein kinase B
dc.subjectprotein p53
dc.subjectunclassified drug
dc.subjectvimentin
dc.subjectX linked inhibitor of apoptosis
dc.subjectArticle
dc.subjectbreast cancer cell line
dc.subjectcancer growth
dc.subjectcarcinogenesis
dc.subjectcell culture
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectDNA synthesis
dc.subjectdown regulation
dc.subjectgene control
dc.subjectgene expression
dc.subjectgene interaction
dc.subjectgene overexpression
dc.subjectgene targeting
dc.subjectgenetic transfection
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmRNA expression assay
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectquantitative analysis
dc.subjectreal time polymerase chain reaction
dc.subjectregulatory mechanism
dc.subjectreverse transcription polymerase chain reaction
dc.subjectRNA extraction
dc.subjectRNA isolation
dc.subjectsignal transduction
dc.subjecttumor suppressor gene
dc.subjectupregulation
dc.subjectWestern blotting
dc.titleMicroRNA-17-5p targets expression of cancer-associated genes in breast cancer cells
dc.typeArticle

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