MicroRNA-17-5p targets expression of cancer-associated genes in breast cancer cells
Yükleniyor...
Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Although there are several studies, biological function and therapeutic potential of miR-17-5p in breast cancer carcinogenesis remains muchly elusive. Specifically, its interaction with several cancer-associated genes remains unexplored in breast cancer. Here, we aimed to demonstrate the biological function and therapeutic potential of miR-17-5p in breast cancer through determination of the interactions between cancer-associated genes and miR-17-5p. MFC-7 breast cancer cells used in the study and cells were transfected with miR-17-5p miRNA mimics to ectopically overexpress miR-17-5p. MiR-17-5p expression levels and expression changes of cancer-associated genes were determined by using qPCR method. Expression levels of miR-17-5p were significantly enhanced following 72 h of mimic transfections as compared to scrambled control and blank control. Overexpression of miR-17-5p led to differential expression of several cancer-associated genes. Notably, BECN, CDKN2B and AIFM genes were significantly altered. Although not significant, expression levels of BAX, BCLXL, VIM, BCL2, MTOR, RB1, AKT1, XIAP, P53, and PTEN genes were found to be slightly elevated whereas expression levels of BCL-2, VIM and AKT1 were found to be decreased. Results of the present study indicate that miR-17-5p can act as both tumor suppressor and tumor promoter oncomiR in breast cancer. © 2019 Elsevier B.V.
Açıklama
Anahtar Kelimeler
AIFM, BECN, Breast cancer, CDKN2B, miR-17-5p, miRNA, apoptosis inducing factor, beclin 1, cyclin dependent kinase inhibitor 2B, mammalian target of rapamycin, microRNA, microrna 17 5p, phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, protein Bax, protein bcl 2, protein bcl xl, protein kinase B, protein p53, unclassified drug, vimentin, X linked inhibitor of apoptosis, Article, breast cancer cell line, cancer growth, carcinogenesis, cell culture, cell proliferation, controlled study, DNA synthesis, down regulation, gene control, gene expression, gene interaction, gene overexpression, gene targeting, genetic transfection, human, human cell, mRNA expression assay, priority journal, protein expression, quantitative analysis, real time polymerase chain reaction, regulatory mechanism, reverse transcription polymerase chain reaction, RNA extraction, RNA isolation, signal transduction, tumor suppressor gene, upregulation, Western blotting
Kaynak
Meta Gene
WoS Q Değeri
N/A
Scopus Q Değeri
Q4
Cilt
24
