Effects of an acetylcholinesterase inhibitor and an N-methyl-D-aspartate receptor antagonist on inflammation and degeneration of the nucleus pulposus

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dc.authoridÖzbek, Hanefi/0000-0002-8084-7855
dc.authoridYILMAZ, Ibrahim/0000-0003-2003-6337
dc.authorscopusid35197435300
dc.authorscopusid57216912962
dc.authorscopusid56769801000
dc.authorscopusid56674583800
dc.authorscopusid57201362754
dc.authorscopusid7005912992
dc.authorwosidÖzbek, Hanefi/O-3472-2019
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorAkalan, Hande
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorKaplan, Nuray
dc.contributor.authorÖzbek, Hanefi
dc.date.accessioned2023-04-20T08:01:11Z
dc.date.available2023-04-20T08:01:11Z
dc.date.issued2022
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentYüksekokullar, Sağlık Yüksekokulu, Beslenme ve Diyetetik Bölümü
dc.description.abstractOBJECTIVE: The study aimed to examine the effects of two drugs, an acetylcholinesterase inhibitor (AChEI) and an N-methyl-D-aspartate receptor (NMDAR) antagonist, on degenerated annulus fibrosus (AF) and nucleus pulposus (NP) cells and the extracellular matrix (ECM) structure in vitro. PATIENTS AND METHODS: Tissue samples were obtained from patients with intervertebral disc herniation (four males and four females; classified as Pfirmann stage IV) and used to prepare cell cultures. Untreated cell culture samples served as the control group. Study group samples were treated with donepezil, memantine or a combination of the two drugs. Cell viability, toxicity and proliferation were evaluated in all groups. Western blotting was used to examine changes in protein expression of signal transducer and activator of transcription 3 (STAT3), phospho-STAT3 (ser727), hypoxia-inducible factor (HIF)-1 alpha (HIF-1 alpha) and nucleotide-binding oligomerisation domain (NOD) leucine-rich repeat (LRR)-containing proteins (NLR) family pyrin domain containing 3 (NLRP3) inflammasome. The alpha significance value was < 0.05. RESULTS: Analysis of the microscopy and commercial kit results revealed that cell proliferation was suppressed. and no cell death was observed. The protein expression levels of NLRP3, STAT3, ser727 and HIF-1 alpha were lower in the samples treated with donepezil and memantine at 72 h (p < 0.05). The protein expression levels of NLRP3, STAT3, ser727 and HIF-1 alpha were higher in the samples treated with the combination of donepezil and memantine (p < 0.05). CONCLUSIONS: The combined administration of memantine a NMDAR antagonist which can prevent neurodegeneration and donepezil an AChEI used for pain relief increased the protein expression levels in the anabolic pathway. However, it did not reduce the protein expression levels in the catabolic pathway. Therefore, further studies are needed to provide extensive insight into whether it may be among the potential targets for the therapy of intervertebral disc (IVD) diseases.
dc.identifier.doi10.26355/eurrev_202206_29080
dc.identifier.endpage4419
dc.identifier.issn1128-3602
dc.identifier.issue12en_US
dc.identifier.pmid35776042
dc.identifier.scopus2-s2.0-85133541368
dc.identifier.scopusqualityQ2
dc.identifier.startpage4409
dc.identifier.urihttps://doi.org/10.26355/eurrev_202206_29080
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10783
dc.identifier.volume26
dc.identifier.wosWOS:000823313800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAkalan, Hande
dc.institutionauthorŞirin, Duygu Yaşar
dc.language.isoen
dc.publisherVerduci Publisher
dc.relation.ispartofEuropean Review For Medical and Pharmacological Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDonepezil
dc.subjectHif-1 Alpha
dc.subjectMemantine
dc.subjectNlrp3
dc.subjectSer727
dc.subjectStat3
dc.subjectIntervertebral Disc Degeneration
dc.subjectNlrp3 Inflammasome
dc.subjectHippocampal Tissue
dc.subjectCells
dc.subjectPathway
dc.subjectActivation
dc.subjectMemantine
dc.subjectApoptosis
dc.subjectDonepezil
dc.subjectProtects
dc.titleEffects of an acetylcholinesterase inhibitor and an N-methyl-D-aspartate receptor antagonist on inflammation and degeneration of the nucleus pulposus
dc.typeArticle

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