A highly sensitive immunosensor based on ITO thin films covered by a new semi-conductive conjugated polymer for the determination of TNF alpha in human saliva and serum samples

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dc.authorid0000-0002-8716-9970
dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidAYDIN, Elif Burcu/ABA-9053-2020
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:10Z
dc.date.available2022-05-11T14:48:10Z
dc.date.issued2017
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA novel, ultrasensitive impedimetric immunosensor was constructed for the detection of tumor necrosis factor alpha (TNF alpha) by using Poly(3-thiophene acetic acid) (P3), a conjugated polymer as an immobilization matrix. The polymer P3 contains a lot of carboxylic acid groups on its surface that provide a larger biorecognition surface. This developed immunosensor was prepared on hydroxy-bearing ITO surface via an ester bond linkage of polymer P3 to immobilize anti-TNF alpha antibodies. The ITO electrode modification steps and interaction between anti-TNF alpha antibody and TNF alpha antigen were monitored by cyclic voltammetry (CV) and by electrochemical impedance spectroscopy (EIS) method. After the analytical parameters optimization, a linear detection response from 0.01 pg/mL to 2 pg/mL, a limit of detection LOD of 3.7 fg/mL and a limit of quantification (LOQ) of 12.4 fg/mL were achieved, which provided accurate results (relative standard deviation; 4.03%). The characterization of this developed immunosensor was performed by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), SEM-energy dispersive X-ray (EDX) mapping and atomic force microscopy (AFM). The immunosensor allowed a simple and fast detection of TNF alpha antigen in human serum and satisfied recoveries (98.69-105.20%) were obtained by using standard addition method.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipThe financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged.
dc.identifier.doi10.1016/j.bios.2017.05.056
dc.identifier.endpage176
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.pmid28599176
dc.identifier.scopus2-s2.0-85020253100
dc.identifier.scopusqualityQ1
dc.identifier.startpage169
dc.identifier.urihttps://doi.org/10.1016/j.bios.2017.05.056
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10607
dc.identifier.volume97
dc.identifier.wosWOS:000405153000025
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Muhammet
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier Advanced Technology
dc.relation.ispartofBiosensors & Bioelectronics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectTumor necrosis factor alpha
dc.subjectLabel free biosensor
dc.subjectElectrochemical impedance spectroscopy
dc.subjectSaliva biomarker
dc.subjectTumor-Necrosis-Factor
dc.subjectLabel-Free
dc.subjectElectrochemical Immunosensor
dc.subjectEarly-Diagnosis
dc.subjectBiosensor
dc.subjectInterleukin-6
dc.subjectAptasensor
dc.subjectBiomarkers
dc.subjectNanoparticles
dc.subjectProgression
dc.titleA highly sensitive immunosensor based on ITO thin films covered by a new semi-conductive conjugated polymer for the determination of TNF alpha in human saliva and serum samples
dc.typeArticle

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