A highly sensitive immunosensor based on ITO thin films covered by a new semi-conductive conjugated polymer for the determination of TNF alpha in human saliva and serum samples
Yükleniyor...
Dosyalar
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Advanced Technology
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel, ultrasensitive impedimetric immunosensor was constructed for the detection of tumor necrosis factor alpha (TNF alpha) by using Poly(3-thiophene acetic acid) (P3), a conjugated polymer as an immobilization matrix. The polymer P3 contains a lot of carboxylic acid groups on its surface that provide a larger biorecognition surface. This developed immunosensor was prepared on hydroxy-bearing ITO surface via an ester bond linkage of polymer P3 to immobilize anti-TNF alpha antibodies. The ITO electrode modification steps and interaction between anti-TNF alpha antibody and TNF alpha antigen were monitored by cyclic voltammetry (CV) and by electrochemical impedance spectroscopy (EIS) method. After the analytical parameters optimization, a linear detection response from 0.01 pg/mL to 2 pg/mL, a limit of detection LOD of 3.7 fg/mL and a limit of quantification (LOQ) of 12.4 fg/mL were achieved, which provided accurate results (relative standard deviation; 4.03%). The characterization of this developed immunosensor was performed by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), SEM-energy dispersive X-ray (EDX) mapping and atomic force microscopy (AFM). The immunosensor allowed a simple and fast detection of TNF alpha antigen in human serum and satisfied recoveries (98.69-105.20%) were obtained by using standard addition method.
Açıklama
Anahtar Kelimeler
Tumor necrosis factor alpha, Label free biosensor, Electrochemical impedance spectroscopy, Saliva biomarker, Tumor-Necrosis-Factor, Label-Free, Electrochemical Immunosensor, Early-Diagnosis, Biosensor, Interleukin-6, Aptasensor, Biomarkers, Nanoparticles, Progression
Kaynak
Biosensors & Bioelectronics
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
97
