The Combined Effect of Melatonin Implant and Removal of Buck Seminal Plasma on Cryopreservation During the Nonbreeding Season

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dc.authoridHuraydin, Oguzhan/0000-0002-4125-6841
dc.authorwosidYILMAZ, Mehmet Melih/GWC-2055-2022
dc.authorwosidHuraydin, Oguzhan/AHB-0614-2022
dc.contributor.authorÜstüner, Burcu
dc.contributor.authorÜstüner, Hakan
dc.contributor.authorGökçe, Elif
dc.contributor.authorÖnder, N. Tekin
dc.contributor.authorYılmaz, M. Melih
dc.contributor.authorHuraydin, Oğuzhan
dc.contributor.authorToker, M. Berk
dc.date.accessioned2023-04-20T08:01:20Z
dc.date.available2023-04-20T08:01:20Z
dc.date.issued2022
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü, Dölerme ve Suni Tohumlama Ana Bilim Dalı
dc.description.abstractThis study aimed to determine how melatonin (MT) and seminal plasma affected the freezability of buck sperm during the nonbreeding season. Semen was collected from eight bucks before (pre-MT) and after (post-MT) MT application in the nonbreeding season. Individual ejaculates were collected from the bucks, split into two equal groups according to the removal of seminal plasma (SP) (-) or nonremoval of SP (+). For washing, the groups of ejaculates were centrifuged, and the supernatant was separated, SP (-) and SP (+) ejaculates were diluted, then frozen. Semen samples were examined for sperm motility, plasma membrane integrity, defective acrosomes, DNA fragmentation, and mitochondrial membrane function at the native and post-thaw stages. When the general average post-thaw motility (p < 0.01), plasma membrane (p < 0.05), acrosome (p < 0.05), and DNA integrity rates (p < 0.05) and mitochondrial membrane potential (MMP) (p < 0.01) were evaluated, it was seen that MT administration caused a statistically significant improvement. The dramatic effect of nonremoval of seminal plasma on motility and plasma membrane integrity is more clearly observed in individual semen samples frozen in the pre-MT group (p < 0.05). Also, it was observed that removing seminal plasma in the post-MT group caused even milder post-thaw acrosome damage compared with the SP (+) group (p < 0.05). The effect of removing seminal plasma was not observed in terms of DNA integrity and MMP rates in pre- and post-MT groups. As a result, it was concluded that MT application and removal of seminal plasma in the nonbreeding season result in improvement in the freezability of buck semen.
dc.identifier.doi10.1089/bio.2022.0074
dc.identifier.issn1947-5535
dc.identifier.issn1947-5543
dc.identifier.pmid36126317
dc.identifier.scopus2-s2.0-85168234980
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1089/bio.2022.0074
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10881
dc.identifier.wosWOS:000855953500001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGökçe, Elif
dc.language.isoen
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofBiopreservation and Biobanking
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBuck Seminal Plasma
dc.subjectMelatonin Implant
dc.subjectCryopreservation
dc.subjectMitochondrial Membrane Potential
dc.subjectDna Integrity
dc.subjectEgg-Yolk
dc.subjectRam Spermatozoa
dc.subjectMembrane Integrity
dc.subjectGoat Spermatozoa
dc.subjectSemen
dc.subjectFreezability
dc.subjectViability
dc.subjectQuality
dc.subjectDeterioration
dc.subjectParameters
dc.titleThe Combined Effect of Melatonin Implant and Removal of Buck Seminal Plasma on Cryopreservation During the Nonbreeding Season
dc.typeArticle

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