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Öğe Dietary intake of Spirulina platensis alters HSP70 gene expression profiles in the brain of rats in an experimental model of mixed stress(Indian Acad Sciences, 2022) Ardıçlı, Sena; Seyidoğlu, Nilay; Köseli, Eda; Gurbanli, Rovshan; Aydın, CenkSpirulina platensis has gradually gained more attention for its therapeutic, antioxidant, and anti-inflammatory potential worldwide. However, the current molecular knowledge about the effects of spirulina on stress-related genes is rather limited. The effects of dietary intake of spirulina on the HSP70 gene expression were assessed in a controlled in vivo experimental design. Moreover, alterations in serum corticosterone levels, IL-2, IL-4, IFN-gamma, triglyceride, ALT, AST, relative gene expression values, and the correlations between them were evaluated. A total of 36 rats were divided into four groups: control group, stress-only group, spirulina group, and spirulina+stress group. To control the dose administration, S. platensis was applied by a gastric gavage in stress groups. Crowded environment stress and hosting alone stress were applied to the stress-only group and spirulina + stress group. RNA was extracted from brain samples using TRlpure and the relative gene expression assessment was performed using Roche-LightCycler-480-II real-time PCR-System. Gene expression values were remarkably different among the four experimental groups. The differences between stress-only and the spirulina groups were statistically significant (P<0.05). The correlation between the HSP70 gene expression and the IFN-gamma was found to be statistically significant (P<0.05; r=0.50). Results indicate a novel effect of spirulina on the HSP70 expression related to the stress-response. Data presented in this study may be useful for further studies to define not only the molecular genetic aspects through dietary S. platensis but also the effects of spirulina on stress-response and animal welfare.Öğe Dose dependent effects of Spirulina platensis on liver health in rats(Agricultural Research Communication Centre, 2020) Seyidoğlu, Nilay; İnan, Sevda; Güneş, NazmiyeSpirulina platensis which is an important microalgae is interested in both human and animal nutrition for health balance. This study's aimed to evaluate the dose dependent effects of S.platensis on body weight, height, waist circumference (WC), body mass index (BMI), waist/height ratio (WHtR), abdominal fat weight, liver weight, serum liver enzymes and liver histopathology. 30 male Wistar albino rats're used in groups; Control, SP-1(500mg/kgbw) and SP-2(1000mg/kgbw). S.platensis was administered by oral gavage daily. The body weight, height, WC, BMI, WHtR and abdominal lipid weight were calculated. Also, serum cholesterol, ALT and AST were measured. End of the study, liver tissue samples were taken and scaled and histopathologic changes were recorded. There were no differences in body conditions, but positively significant correlations were found among parameters. As the doses were considered, in groupSP-1, significant positively correlations were determined among abdominal fat, growth, height, BMI and WC statistically. Nevertheless, there were no significant changes in liver weight, serum and histopathologic analyzes. S.platensis with its high concentration of functional nutrients is called as an important alternative therapeutic food and it can be used safely. © 2020 Agricultural Research Communication Centre. All rights reserved.Öğe Mutual Living with Pomacea maculata and Catfish and Their Benefits for Feeding(2023) Seyidoğlu, Nilay; Yağcılar, Çetin; Karakcı, DenizPomacea snails have been interested around the world due to their rich biological contents. In this study, we assessed the living condition and feeding strategies of catfish (Loricariidae) with Pomacea maculata. We used thirty catfish and ten P.maculata in three replicates for four months. There were two groups as: Control and P. maculata (catfish and P.maculata together). The body weights and lengths of catfish were measured monthly. Also feeding behavior of catfish was recorded during the trial. There were significant increases determined in last weight, total weight gain, last body length, and total body length gain in the Pomacea group compared to Control. It was observed that catfish in the Pomacea group ate the food which was lumped on the mucous of P.maculata. These results suggested that due to this feeding behavior of catfish, they had good progress. It can be concluded that P.maculata can be beneficial for ornamental fisheries.Öğe Oxidative stress in dogs during mid-gestation abortion: Fetal and maternal antioxidant alterations with clinical and hematological features(Hellenic Veterinary Medical Soc, 2022) Özalp, G. R.; Gül, Z.; Seyidoğlu, Nilay; Büyüküysal, L.The present study aimed to evaluate the GSH and MDA levels in dog???s serum and vital organs of aborted fetuses, after application of two abortifacient. Animals of group I (n = 6) were treated twice with subcutaneous injection of 10 mg/kg aglepristone and group II (n = 3) received prostaglandin F2?? at a dose of 125 ??g/kg every 12 hours until abortion process completed. Hematologic examinations and sedimentation rates were performed of bitches. The duration between the first occurrence of vaginal discharge to first expulsion of fetuses ranged between 14-29 hours (mean: 22.6??5.5 h) and 24-33 hours in group I and II, respectively. Both treatments significantly increased the serum MDA levels (P<0.01); however aglepristone increased the MDA levels throughout the study, prostaglandin enhanced plasma MDA levels only on day 1. GSH levels were significantly lower in aglepristone-treated bitches than prostaglan-din-treated animals (P 0.05). The treatments resulted in similar MDA levels in liver and heart tissues (P 0.05). GSH levels in kidney, muscle and heart tissues did not differ between groups (P>0.05), aglepristone resulted reduced GSH levels in liver tissue (P<0.05). High MDA levels could be evaluated as a useful marker for fetal suffering in pregnancy controls. The MDA and GSH levels, measured in vital organs of fetuses, could suggest possible toxic effects of aborti-facients in newborns, as both medical agents are being used in parturition induction.Öğe The Anesthetic Role of Some Herbal Oils for Zebrafish(2020) Seyidoğlu, Nilay; Yağcılar, ÇetinAnesthetics have been important in ornamental fishes for transport and restriction of behaviors as well as for surgical applications and studies in science. Also, more economical and appropriate anesthetics can be common choice for these stiuations. In the study, a total of two hundred and fifty two zebrafish (forty two females and forty two males in each group) divided in three groups were studied as group A (Anise oil); group T (Thyme oil); group M (Mint oil). Anesthesia intake and lethal dose values were determined by following the anesthetic entry and recovery times. There was no anesthetic effect in group A, while anesthetic effects occured in groups T and M in concentrations 1, 5, 10, 20 and 30 mg/l. Similarity was found in groups T and M in terms of anesthesia entry and recovery times (P>0.05). Nevertheless, no mortality occured in group M. However, 5mg/l tyme oil anesthesia was determined to cause 50% mortality in male fishes (group T). It was also observed that mint oil has an anesthetic effect on zebrafish with increa-sing doses determined by anesthesia entry and recorvery times (group M, P<0.05). Thereby, mint oil may be an alter-native herbal anesthetic agent for zebrafish. However, further studies are neccessary to show the herbal anesthetics effect on ornamental fishes.Öğe The effects of Spirulina platensis (Arthrospira platensis) and Saccharomyces cerevisiae on the distribution and cytokine production of CD4+and CD8+T-lymphocytes in rabbits(Univ Austral Chile, Fac Ciencias Veterinarias, 2017) Seyidoğlu, Nilay; Galip, Nurten; Budak, Ferah; Uzabacı, EnderNatural additives have become one of the most alternative immune enhancer nowadays. In particular, Spirulina platensis (Arthrospira platensis) (SP) and Saccharomyces cerevisiae (SC) have been used for improving the immune system and quality of life. The aim of this study was to regulate the immune effect of S. cerevisiae and S. platensis (A. platensis) combination. Forty male New Zealand white rabbits, aged 5-6 weeks, were analysed in 4 groups: Control (basal diet); SC (added 3 g/kg diet), SP (added 5% of the diet); SC and SP (added 3 g/kg SC and 5% SP of the diet). The entire experiment lasted 90 days. Blood samples were obtained by ear venipuncture on the 90th day. The CD4+ and CD8+ T lymphocyte values were determined by flow cytometry and cytokines (IFN-gamma and IL-4) were determined by ELISA. According to the results, there were no significant differences in the expression of cytokines, but serum CD4+/CD8+ increased in the animals fed SP and SC+SP supplemented diets (3 g/kg and 5% of the diet, respectively). It was concluded that S. platensis (A. platensis) may be used as an immune enhancer, although further studies are needed to clarify the effects of spirulina supplement on immunity.Öğe The influence of phytogenic additive on the antioxidant capacity, immunity and liver functions in stress-induced male rats(Hellenic Veterinary Medical Soc, 2022) Köşeli, E.; Seyidoğlu, Nilay; Gurbanlı, R.; Aydın, C.Global animal feeding strategies have been modified due to several ecosystem changes that cause stress and decline in health, growth, and yields.According to the literature, natural additives have been fundamental to animal health from day to day. The present study measured changes in antioxidant status, immunity, liver functions and organ weights tested under stress conditions to determine whether dietary supplementation with phytogenic additive could provide beneficial effects. Forty-eight adult,male Sprague-Dawley rats were randomly separated into four groups;-Control(C),Stress(S),Treatment(Tr),Treatment and Stress(TrS). Rats in groups Tr and TrS received phytogenic additive by adding into water (2mL/L) 5days a week for 28days. All rats were exposed to prolonged light phase conditions (18h light: 6h dark) for 14 days. Also, two chronic stresses, isolation and crowded environments, were applied to animals in the Stress and TrS groups. There was a significant decline in the oxidant status in untreated stress group, while phytogenic additive fed rats maintained a significantly higher total antioxidant status. This study also showed a significant increase in IL-4 and decrease in IFN-gamma in the untreated Stress group compared to the Control group. There were increases in liver enzymes in the Stress group in comparison to the Control group. After the phytogenic treat-ment, there was an increase in the weight of the liver, intestine, brain and testes. In conclusion, this study showed that supplementation of phytogenic additive containing milk thistle and artichoke with choline,carnitine, vitamin E and methionine describes the protective effects on antioxidant status, immunological parameters and liver functions under mixed stress conditions.Öğe The prevention role of Spirulina platensis (Arthrospira platensis) on intestinal health(Elsevier B.V., 2022) Asmaz, E.D.; Seyidoğlu, NilaySpirulina is a popular herbal food that has a preventive effect on health. In the study, it aimed to evaluate the effects of different doses of Spirulina platensis (Arthrospira platensis) on the morphological properties of the duodenum of rats, and to assign the effect of Spirulina on the expression of proliferating cell nuclear antigen (PCNA) in the rat duodenum, and thereby to observe the effects on intestinal health. 30 male Wistar albino rats were divided into 3 groups for 45 days. The first group was received the basal diet; the second group was given 500 mg/kg Spirulina daily by oral gavage; the third group was given 1000 mg/kg Spirulina daily. The duodenum segments were taken at the end of the trial and processed for histological assay. Although the total mucosa, villus height and villus/cript ratio were found higher in high dose Spirulina, the lower cript depth was detected in same group compared to control and low dose group. A significant increase was observed at high dose compared to the control and low dose group in terms of PCNA expression intensity and proliferation index. Findings suggest that high dose of Spirulina may support the duodenal growth, and thereby intestinal health. © 2022Öğe The preventive role of Spirulina platensis (Arthrospira platensis) in immune and oxidative insults in a stress-induced rat model(Sciendo, 2021) Seyidoğlu, Nilay; Köşeli, Eda; Gurbanli, Rovshan; Aydın, CenkIntroduction: There is a balance between oxidative stress, antioxidant capacity and immune response. Their roles in physiological and behavioural mechanisms are important for the maintenance of the organism's internal equilibrium. This study aimed to evaluate the antioxidant effects of the exogenous alga Spirulina platensis (Arthrospira platensis) in a stress-induced rat model, and to describe its possible mechanism of action. Material and Methods: Thirty-six adult male Sprague Dawley rats were separated into four groups: Control (C), stress (S), S. platensis (Sp), and S. platensis + stress (SpS). The rats in groups Sp and SpS were fed with 1,500 mg/kg b.w./day Spirulina platensis for 28 days. All rats were exposed to prolonged light phase conditions (18 h light : 6 h dark) for 14 days. The SpS and S groups were exposed to stress by being kept isolated and in a crowded environment. Blood samples were obtained by puncturing the heart on the 28th day. The effect of stress on serum corticosterone, oxidative stress markers (TOS, TAC, PON1, OSI) and immunological parameters (IL-2, IL-4, IFN-?) were tested. Also, the brain, heart, intestines (duodenum, ileum, and colon), kidney, liver, spleen, and stomach of the rats were weighed. Results: Serum corticosterone levels were higher in the S group than in the C group, and significantly lower in the SpS group than in the S group. Mean total antioxidant capacity were lower in the S group than in the C group, and Spirulina reversed this change. Although not significantly different, IL-2 was lower in the S group than in the C group. However, in the SpS group, IL-2 increased due to Spirulina platensis mitigating effects of stress. Conclusion: Male rats fed a diet with Spirulina platensis could experience significantly milder physiological changes during stress, although stress patterns may be different. Exogenous antioxidant supplements merit further investigation in animals and humans where the endogenous defence mechanism against stress may not be sufficient. © 2021 Sciendo. All rights reserved.Öğe The relationship between Spirulina platensis and selected biomechanical indicators of tibiae in rats(Czech Academy of Agricultural Sciences, 2020) Süzer, Bayram; Seyidoğlu, Nilay; Tüfekci, Kenan; İnan, SevdaThere are several dietary supplements, particularly herbal foods, that have been used in an attempt to improve bone growth. In this study, we aim at determining the effects of low- and high-doses of Spirulina platensis, a “Superfood”, on the bone growth and biomechanical indicators. Thirty Wistar rats, weighing 250 g, at the age of 7–8 weeks were assigned to three groups: The Control group (basal diet), Low-dose group (LDG; 500 mg/kg) and High-dose group (HDG; 1 000 mg/kg) of S. platensis. S. platensis was given daily by oral gavage in a 45-day-trial. At the end of the study, the right tibiae were collected and subjected to bone biomechanical tests (bone weight, bone length, maximum load, stiffness, breaking deflection, fracture toughness, post-yield displacement and yield load). Serum samples were also analysed for the calcium and phosphorus concentrations. There were significant increases in bone weight, bone length, maximum load, breaking deflection, work to fracture, post-yield displacement and yield load (P = 0.025, P = 0.019, P = 0.030, P = 0.015, P = 0.031, P = 0.028, P = 0.049, respectively), whereas stiffness non-significantly increased. However, there were no significant differences (P > 0.05) for any variables between the LDG and the HDG. Although the serum phosphorus concentrations showed no differences among any of the groups, the serum calcium concentration increased significantly in LDG compared to Control group (P=0.009; 7.14 ± 0.47 and 9.45 ± 0.67, respectively). However, no differences were observed in HDG in terms of serum calcium. In conclusion, S. platensis had positive effects on the bone growth and biomechanical bone features. Therefore, our study supports the use of S. platensis as an alternative food additive for bone growth and health in growing animals. © 2020 Czech Academy of Agricultural Sciences. All rights reserved.
