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Öğe Effects of Thymoquinone Supplementation on Somatostatin Secretion in Pancreas Tissue of Rats(Kafkas Univ, Veteriner Fakultesi Dergisi, 2017) Bakır, Buket; Karadağ Sarı, Ebru; Eliş Yıldız, Sevda; Asker, HasanIn this study, it was aimed to investigate the effects of thymoquinone (TQ), which is the bioactive phytochemical constituent of the seeds oil of Nigella sativa, on somatostatin secretion in the rat pancreatic tissue by immunohistochemical method. Animals (n=30) were divided into 3 groups as follows: control, sham and thymoquinone. While Thymoquinone group received 8 mg/kg of TQ which injected for 14 days, only isotonic saline was injected to the sham group for same time. The control group received nothing. Crossman's triple staining was applied to tissue sections to examine histology. Streptavidin-Biotin-Peroxidase Complex method was used to investigate somatostatin immunoreactivity in the pancreatic tissue. Specific somatostatin immunoreactivities were observed in endocrine cells (islets of Langerhans) in all groups. It was determined that somatostatin secretion increased in the thymoquinone group compared to control and sham. In conclusion, thymoquinone administration was concluded to increase the secretion of somatostatin that is known to regulate certain hormones such as growth hormone, insulin, glucagon, secretin and gastrin.Öğe Immunohistochemical Distribituon of COX-1, COX-2, and TGF beta-1 in the Duodenum of Rats Treated with Capsaicin(Kafkas Univ, Veteriner Fakultesi Dergisi, 2016) Bakır, Buket; Eliş Yıldız, Sevda; Karadağ Sarı, Ebru; Asker, Hasan; Nazli, MumtazThe purpose of this study was to investigate the effects of capsaicin on the duodenal distribution of cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and Transforming growth factor beta-1 (TGF beta-1) in rats. Rats were divided into two groups (n = 10 in each group). Experimental group treated with capsaicin (1 mg/kg, subcutaneously) was injected in 10% ethanol, 1% Tween and 80% distilled water everyday for a period of one week and in not injected control group. Localisations of COX-1, COX-2 and TGF beta-1 were observed in villus and crypt ephitelial cells, and smooth muscle cells. In conclusion, in this study, capsaicin administration was found to increase the release of COX-1 and COX-2, and TGF beta-1.Öğe Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice(Ankara Univ Press, 2017) Bakır, Buket; Erdag, Dinçer; Eliş Yıldız, Sevda; Karadağ Sarı, Ebru; Asker, Hasan; Sozmen, MahmutThe purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by malathion that is used as an insecticide in agriculture, on catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in kidney tissues by immunohistochemical technique. A total of 48 male mice (Mus musculus) were used in our study. Mice were divided 6 groups (control group, maize oil group, normal saline group, Onosma nigricaule group, malathion group, Onosma nigrcaule+malathion group). Hematoxylin-eosin and triple staining methods were used for histological and pathological examinations. The localization of CAT and Mn-SOD in the renal tissue was determined using the method of streptavidinbiotin- peroxidase. CAT immunoreactivity was determined with a weak intensity in epithelium of renal tubulus proximalis of mice in the malathion group, with a moderate intensity in Onosma nigricaule+ malathion group and with a higher intensity in tubulus proximalis of other groups. A cytoplasmic Mn-SOD immunoreactivity was determined with weak intensity in renal medulla of mice in malathion group, with moderate intensity in renal medulla of mice in Onosma nigricaule plant extract+ malathion group, maize oil group, and normal saline group and with highly intensity in control and Onosma nigricaule groups. It was concluded that Onosma nigricaule might play a protective role as an antioxidant against the oxidant features of malathion.Öğe Malathion ve Onosma nigricaule (Boraginaceae)’nin Fare Böbrek Dokusunda Leptin Üzerine Etkilerinin İmmunohistokimyasal Olarak İncelenmesi(Namık Kemal Üniversitesi, Tıp Fakültesi, 2015) Bakır, Buket; Yıldız, Sevda Eliş; Erdağ, Dinçer; Sözmen, Mahmut; Asker, HasanAmaç Bu çalışmada, tarımda bir insektisit olarak kullanılan malathion’un neden olduğu oksidasyon parametreleri üzerine Onosma nigricaule (Boraginaceae)’den elde edilen bitkisel özütün böbrek dokusunda leptin üzerine etkisinin immunohistokimyasal olarak araştırılması amaçlandı. Materyal-Metot Çalışmamızda, toplam 48 adet erkek fare kullanıldı. Gruplar; kontrol (grup 1), malathion’un taşıt maddesi olan mısır yağı (grup 2), bitki özütünün taşıt maddesi olan serum fizyolojik (grup 3), malathion (grup 4), Onosma nigricaule (grup 5), ve Onosma nigricaule + malathion grubu (grup 6) olmak üzere toplam 6 grup olarak belirlendi. Böbreklerden alınan kesitlere leptin immunoreaktivitesini belirlemek için Streptavidin-Biotin-Peroksidaz Kompleks tekniği uygulandı. Hazırlanan kesitlere histolojik incelemeler için hematoksilen-eosin ve Crossman’ın üçlü boyama yöntemi kullanıldı. Bulgular Kontrol grubu ile taşıt kontrol grupları olan serum fizyolojik grubu ve mısır yağı grupları ile Onosma nigricaule, malathion ve Onosma nigricaule + malathion gruplarının böbrek dokularında herhangi bir lezyon tespit edilmedi. Diğer yandan, kontrol, mısır yağı, serum ve malathion grubundaki farelerin tubulus proksimalis ve tubulus distalis epitel hücrelerinde güçlü leptin immunoreaktivite görülmesine karşın Onosma nigricaule ve Onosma nigricaule + malathion grubu farelerde ise zayıf leptin immunoreaktivitesi tespit edildi. Sonuç Fareler ile yaptığımız çalışmada, yurdumuzda insektisit olarak yaygın kullanılan malathion’un fare böbreğinde leptin aktivitelerini etkilediği görülmüştür.Öğe MALATHİON VE ONOSMA NİGRİCAULE (BORAGİNACEAE)’NİN FARE BÖBREK DOKUSUNDA LEPTİN ÜZERİNE ETKİLERİNİN İMMUNOHİSTOKİMYASAL OLARAK İNCELENMESİ(Tekirdağ Namık Kemal Üniversitesi, 2019) Bakır, Buket; Yıldız, Sevda Eliş; Erdağ, Dinçer; Sözmen, Mahmut; Asker, Hasan[Abstract Not Available]Öğe The Eff ects of the Basil (Ocimum sanctum) Treatment on the Tumor Necrosis Factor-? and Interleukin 1? Release in the Kidney Tissue of the Diabetic Rats(Kafkas Univ, Veteriner Fakultesi Dergisi, 2021) Eliş Yıldız, Sevda; Bakır, Buket; Asker, Hasan; Karadağ Sarı, EbruThis study aims to examine the changes of the Ocimum sanctum treatment on the tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) in the kidney tissue of the rats, in which the experimental diabetes was induced with streptozotocin (STZ). Forty Sprague Dawley male rats were divided into 5 groups: Diabetes, Diabetes + Ocimum sanctum, Ocimum sanctum, Control, and Sham. The immunohistochemical localization of TNF-alpha and IL-1 beta in the kidney tissue was determined by using the streptavidin-biotin-peroxidase method. Strong TNF-alpha immunoreactivity was determined in the renal cortex of the rats in the Diabetes and Diabetes + Ocimum sanctum groups on 14th days, low immunoreactivity was determined in the rats in Ocimum sanctum, Sham, and Control groups. While strong IL-1 beta immunoreactivity was observed in the renal cortex of the Diabetes group, moderate IL-1 beta immunoreactivity was observed in the renal cortex of the Diabetes + Ocimum sanctum and low immunoreactivity was determined in the Ocimum sanctum, Sham, and Control groups. In this study, it was assessed how the polymorphisms, occurring in the cytokine genes of Ocimum sanctum in the rats, in which experimental diabetes was induced, and TNF-alpha and IL-1 beta, which was demonstrated to have an important role in the complication development in the diabetic patients affected the renal tissue.Öğe The structure of spleen in the long-legged buzzard (Buteo rufinus): Histological and immunohistochemical study(Wiley, 2022) Asker, Hasan; Bakır, Buket; Kurtdede, Nevin; Altunay, Hikmet; Sarı, Ebru KaradağThe aim of this study was to examine the structure of the spleen in the long-legged buzzard (Buteo rufinus) by histologically and the distribution of the catalase in the spleen by immunohistochemically. The tissue materials were taken from three healthy long-legged buzzards provided by permission of the General Directorate of Nature Protection and National Parks (Ankara, Turkey). The long-legged buzzard spleen is surrounded by smooth muscle cells and fibrous connective tissue. In addition, trabecular structures are not found in the capsule. The separation of white and red pulp areas could not be precisely determined. Plasma cells were detected especially around the lymphoid follicles and lymphatic follicles and Schweigger-Seidel cells were formed by reticulum strands. Alcian blue positive reaction was determined in Schweigger-Seidel cells and endothelial cells of the long-legged buzzard spleen. The intense cytoplasmic catalase immunoreactivity was observed in the Schweigger-Seidel cells and nuclear in some red pulp cells of the long-legged buzzard spleen. Consequently, determination of the histological structure and catalase immunoreactivity of the spleen on the long-legged buzzard by this study could be support for the future researches.
