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The fate of 35S rRNA genes in the allotetraploid grass Brachypodium hybridum

dc.contributor.authorBorowska-Zuchowska, N.
dc.contributor.authorKovarik, A.
dc.contributor.authorRobaszkiewicz, E.
dc.contributor.authorTuna, Metin
dc.contributor.authorTuna, G.S.
dc.contributor.authorGordon, S.
dc.contributor.authorHasterok, R.
dc.date.accessioned2022-05-11T14:07:14Z
dc.date.available2022-05-11T14:07:14Z
dc.date.issued2020
dc.identifier.issn0960-7412
dc.identifier.urihttps://doi.org/10.1111/tpj.14869
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5019
dc.description.abstractNucleolar dominance (ND) consists of the reversible silencing of 35S/45S rDNA loci inherited from one of the ancestors of an allopolyploid. The molecular mechanisms by which one ancestral rDNA set is selected for silencing remain unclear. We applied a combination of molecular (Southern blot hybridization and reverse-transcription cleaved amplified polymorphic sequence analysis), genomic (analysis of variants) and cytogenetic (fluorescence in situ hybridization) approaches to study the structure, expression and epigenetic landscape of 35S rDNA in an allotetraploid grass that exhibits ND, Brachypodium hybridum (genome composition DDSS), and its putative progenitors, Brachypodium distachyon (DD) and Brachypodium stacei (SS). In progenitor genomes, B. stacei showed a higher intragenomic heterogeneity of rDNA compared with B. distachyon. In all studied accessions of B. hybridum, there was a reduction in the copy number of S homoeologues, which was accompanied by their inactive transcriptional status. The involvement of DNA methylation in CG and CHG contexts in the silencing of the S-genome rDNA loci was revealed. In the B. hybridum allotetraploid, ND is stabilized towards the D-genome units, irrespective of the polyphyletic origin of the species, and does not seem to be influenced by homoeologous 35S rDNA ratios and developmental stage. © 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.en_US
dc.description.sponsorshipU.S. Department of Energy, USDOE: DE?AC02?05CH11231; Office of Science, SC; Grantová Agentura ?eské Republiky, GA ?R: 20-14133J; Narodowe Centrum Nauki, NCN: 2018/02/X/NZ3/03518, 2018/31/B/NZ3/01761en_US
dc.description.sponsorshipWe thank Dr Glyn Jenkins (Aberystwyth University, UK) for his valuable comments on the article. The authors gratefully acknowledge financial support from the National Science Centre, Poland (grant no. 2018/31/B/NZ3/01761 and 2018/02/X/NZ3/03518) and the Czech Science Foundation (20-14133J). The work conducted by the US DOE Joint Genome Institute is supported by the Office of Science of the US Department of Energy under contract no. DE-AC02-05CH11231.en_US
dc.description.sponsorshipWe thank Dr Glyn Jenkins (Aberystwyth University, UK) for his valuable comments on the article. The authors gratefully acknowledge financial support from the National Science Centre, Poland (grant no. 2018/31/B/NZ3/01761 and 2018/02/X/NZ3/03518) and the Czech Science Foundation (20?14133J). The work conducted by the US DOE Joint Genome Institute is supported by the Office of Science of the US Department of Energy under contract no. DE?AC02?05CH11231.en_US
dc.language.isoengen_US
dc.publisherBlackwell Publishing Ltden_US
dc.identifier.doi10.1111/tpj.14869
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject35S rDNA evolutionen_US
dc.subject35S rRNA gene expressionen_US
dc.subjectallopolyploidyen_US
dc.subjectBrachypodium hybridumen_US
dc.subjectnucleolar dominanceen_US
dc.subjectAlkylationen_US
dc.subjectFluorescence microscopyen_US
dc.subjectPlants (botany)en_US
dc.subjectRNAen_US
dc.subjectCleaved amplified polymorphic sequencesen_US
dc.subjectDevelopmental stageen_US
dc.subjectFluorescence in situ hybridizationen_US
dc.subjectMolecular mechanismen_US
dc.subjectNucleolar dominancesen_US
dc.subjectPolyphyletic originen_US
dc.subjectReverse transcriptionen_US
dc.subjectSouthern blot hybridizationen_US
dc.subjectTranscriptionen_US
dc.subjectAlkylationen_US
dc.subjectGenesen_US
dc.subjectHybridizationen_US
dc.subjectNucleic Acidsen_US
dc.subjectRatiosen_US
dc.subjectReductionen_US
dc.subjectSeten_US
dc.subjectBrachypodiumen_US
dc.subjectcopy number variationen_US
dc.subjectDNA methylationen_US
dc.subjectgene locusen_US
dc.subjectgenetic polymorphismen_US
dc.subjectgeneticsen_US
dc.subjectmetabolismen_US
dc.subjectmolecular evolutionen_US
dc.subjectplant chromosomeen_US
dc.subjectplant geneen_US
dc.subjectplant genomeen_US
dc.subjectRNA geneen_US
dc.subjectSouthern blottingen_US
dc.subjecttetraploidyen_US
dc.subjectBlotting, Southernen_US
dc.subjectBrachypodiumen_US
dc.subjectChromosomes, Planten_US
dc.subjectDNA Copy Number Variationsen_US
dc.subjectDNA Methylationen_US
dc.subjectEvolution, Molecularen_US
dc.subjectGenes, Planten_US
dc.subjectGenes, rRNAen_US
dc.subjectGenetic Locien_US
dc.subjectGenome, Planten_US
dc.subjectPolymorphism, Geneticen_US
dc.subjectTetraploidyen_US
dc.titleThe fate of 35S rRNA genes in the allotetraploid grass Brachypodium hybridumen_US
dc.typearticleen_US
dc.relation.ispartofPlant Journalen_US
dc.departmentFakülteler, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.identifier.volume103en_US
dc.identifier.issue5en_US
dc.identifier.startpage1810en_US
dc.identifier.endpage1825en_US
dc.institutionauthorTuna, Metin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.authorscopusid57191574880
dc.authorscopusid7004490346
dc.authorscopusid56979278500
dc.authorscopusid57225437022
dc.authorscopusid57189634048
dc.authorscopusid56371114900
dc.authorscopusid57205373877
dc.identifier.wosWOS:000545082000001en_US
dc.identifier.scopus2-s2.0-85087457230en_US
dc.identifier.pmid32506573en_US


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