A label-free and sensitive impedimetric immunosensor for TNF ? biomarker detection based on epoxysilane-modified disposable ITO-PET electrode
Özet
The present study constructed a simple and cost-effective electrochemical immunosensor based on 3-glycidoxypropyltriethoxysilane (GPTES) monolayer coated indium tin oxide-polyethylene terephthalate (ITO-PET) electrode for selective and sensitive determination of Tumour necrosis factor ? (TNF ?) cancer biomarker. GPTES was a well-known surface coupling silane agent which had epoxy groups to bind antibodies. Anti-TNF ? antibodies were chosen as biorecognition elements and they attached covalently on the GPTES modified ITO-PET electrode. Electrochemical determination of immunoreaction between anti-TNF ? antibodies and TNF ? antigens was the basic principle of the proposed immunosensor. The electrochemical characterisation of the designed biosensor was tested with electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. In addition, the surface morphological characteristics of the ITO-PET electrodes after several modification stages were monitored by scanning electron microscopy (SEM) and atomic force microscopy (AFM) imaging. The analytical success of the proposed immunosensor was tested with respect to linear detection range, detection limit, repeatability, reproducibility, reusability, regeneration and long-term stability. The present biosensor had a wide linear range from 0.01 to 1.5 pg/mL and a low detection limit of 3.1 fg/mL. Finally, the biosensor successfully detected TNF ? cancer biomarker in human serum samples with high recovery ranges. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
Cilt
100Sayı
4İlgili Öğeler
Başlık, yazar, küratör ve konuya göre gösterilen ilgili öğeler.
-
A comparison between LP(GMA) and CLP(GMA) polymer composites as an immobilization matrix for biosensing applications: A model immunosensor for IL 1a
Aydın, Elif Burcu; Sezgintürk, Mustafa Kemal (Elsevier B.V., 2019)A comparative study was made by using two matrices (Linear poly(glycidyl methacrylate) (GMA) polymer (LP(GMA)) and carbon black-polyvinylidene fluoride (PVDF)-LP(GMA) composite CLP(GMA)) as immobilization platforms for ... -
A novel electrochemical immunosensor based on acetylene black/epoxy-substituted-polypyrrole polymer composite for the highly sensitive and selective detection of interleukin 6
Aydın, Elif Burcu; Aydın, Muhammet; Sezgintürk, Mustafa Kemal (Elsevier B.V., 2021)An ultrasensitive immunosensor based on acetylene black (AB)/epoxy-substituted-poly(pyrrole) polymer (EpxS-PPyr) composite coated disposable indium tin oxide (ITO) electrode was fabricated for interleukin 6 (IL 6) detection. ... -
Fabrication of electrochemical immunosensor based on acid-substituted poly(pyrrole) polymer modified disposable ITO electrode for sensitive detection of CCR4 cancer biomarker in human serum
Aydın, Elif Burcu; Aydın, Muhammet; Sezgintürk, Mustafa Kemal (Elsevier B.V., 2021)This study described the first impedimetric immunosensor reported for the determination of CCR4, a new prostate cancer biomarker. This impedimetric immunosensor was constructed through the modification of disposable indium ...