Recent advances of structure, function, and engineering of carboxylesterases for the pharmaceutical industry: A minireview

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dc.contributor.authorSurmeli, Yusuf
dc.contributor.authorVardar-Yel, Nurcan
dc.contributor.authorTutuncu, Havva Esra
dc.date.accessioned2025-04-06T12:23:53Z
dc.date.available2025-04-06T12:23:53Z
dc.date.issued2025
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractCarboxylesterases have a wide range of applications due to their catalytic efficiency, robust structure, and broad substrate specificity. These enzymes, which can hydrolyze carboxylic acid esters, amides, and thioesters, stand out with their regio- and enantioselective properties. They play a crucial role in synthesizing pharmaceutical intermediates, including secondary and tertiary alcohols, alpha-hydroxy acids, and various bioactive compounds. However, in some cases, the enantioselectivity of carboxylesterases may be insufficient to achieve conversions with the purity required by the pharmaceutical industry. This review summarizes the crucial role of carboxylesterases, particularly in the pharmaceutical field, focusing on the classification, structure, and engineering approaches. After introducing the main families of carboxylesterases, the structural studies are presented to give a comprehensive insight into the active site architecture and related key determinants for enantioselectivity. The protein engineering studies to improve the enantioselectivity of carboxylesterases are discussed along with solvent engineering and immobilization applications.
dc.identifier.doi10.1016/j.ijbiomac.2025.142206
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid40107535
dc.identifier.scopus2-s2.0-105000121216
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2025.142206
dc.identifier.urihttps://hdl.handle.net/20.500.11776/17234
dc.identifier.volume307
dc.identifier.wosWOS:001450802100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryDiğer
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250406
dc.subjectCarboxylesterase
dc.subjectPharmaceuticals
dc.subjectEnantioselectivity
dc.subjectEngineering strategies
dc.subjectalpha/(3-Hydrolase fold
dc.subjectCap domain
dc.titleRecent advances of structure, function, and engineering of carboxylesterases for the pharmaceutical industry: A minireview
dc.typeReview

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